CRISPR-U™ Gene Knockout Cell Line Strategy

PAK2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PAK2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PAK2
Gene id 5062
Organism Homo sapiens
Official full symbol p21 (RAC1) activated kinase 2
Gene type protein-coding
Also known as PAK65, PAKgamma
Summary The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
Genomic regions Chromosome 3
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PAK2-201 ENST00000327134.7 6139 524aa Protein coding CCDS3321 Q13177 NM_002577.4 TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92,
PAK2-202 ENST00000426668.1 689 221aa Protein coding - H7C1X3 - CDS 5' incomplete, TSL:3,
PAK2-203 ENST00000481344.1 371 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PAK2
This KO Strategy loading
Red Cotton™ Notes Gene PAK2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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