CRISPR-U™ Gene Knockout Cell Line
Strategy
IL20 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IL20 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IL20 |
| Gene id | 50604 |
| Organism | Homo sapiens |
| Official full symbol | interleukin 20 |
| Gene type | protein-coding |
| Also known as | IL-20, IL10D, ZCYTO10 |
| Summary | The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IL20-202 | ENST00000367098.6 | 1284 | 176aa | Protein coding | CCDS1470 | Q9NYY1-1 | NM_018724.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IL20-201 | ENST00000367096.7 | 925 | 176aa | Protein coding | CCDS1470 | Q9NYY1-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| IL20-203 | ENST00000391930.3 | 1153 | 151aa | Protein coding | - | Q9NYY1-2 | - | TSL:1, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IL20 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IL20 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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