CRISPR-U™ Gene Knockout Cell Line
Strategy
PAK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PAK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PAK1 |
| Gene id | 5058 |
| Organism | Homo sapiens |
| Official full symbol | p21 (RAC1) activated kinase 1 |
| Gene type | protein-coding |
| Also known as | IDDMSSD, PAKalpha, alpha-PAK, p65-PAK |
| Summary | This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PAK1-202 | ENST00000356341.8 | 3459 | 545aa | Protein coding | CCDS8250 | Q13153-1 | NM_002576.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PAK1-201 | ENST00000278568.8 | 2543 | 553aa | Protein coding | CCDS44687 | Q13153-2 | - | TSL:2, GENCODE basic, |
| PAK1-215 | ENST00000530617.5 | 3001 | 522aa | Protein coding | - | B3KNX7 | - | TSL:2, GENCODE basic, |
| PAK1-210 | ENST00000528203.5 | 1878 | 455aa | Protein coding | - | E9PM17 | - | TSL:2, GENCODE basic, |
| PAK1-218 | ENST00000533285.1 | 878 | 244aa | Protein coding | - | H0YCG5 | - | CDS 5' incomplete, TSL:3, |
| PAK1-211 | ENST00000528592.5 | 581 | 89aa | Protein coding | - | E9PMP2 | - | CDS 3' incomplete, TSL:3, |
| PAK1-213 | ENST00000529248.5 | 576 | 106aa | Protein coding | - | E9PKH9 | - | TSL:4, |
| PAK1-203 | ENST00000524847.5 | 559 | 99aa | Protein coding | - | E9PQW5 | - | CDS 3' incomplete, TSL:5, |
| PAK1-207 | ENST00000526968.1 | 502 | 26aa | Protein coding | - | E9PJF8 | - | CDS 3' incomplete, TSL:4, |
| PAK1-212 | ENST00000528633.1 | 450 | 63aa | Protein coding | - | E9PRP6 | - | CDS 3' incomplete, TSL:3, |
| PAK1-208 | ENST00000527457.5 | 1047 | 167aa | Nonsense mediated decay | - | H0YCM0 | - | CDS 5' incomplete, TSL:3, |
| PAK1-209 | ENST00000527535.5 | 576 | 18aa | Nonsense mediated decay | - | H0YF55 | - | CDS 5' incomplete, TSL:5, |
| PAK1-204 | ENST00000525542.5 | 863 | No protein | Processed transcript | - | - | - | TSL:2, |
| PAK1-217 | ENST00000532991.5 | 806 | No protein | Retained intron | - | - | - | TSL:3, |
| PAK1-220 | ENST00000533568.5 | 762 | No protein | Retained intron | - | - | - | TSL:3, |
| PAK1-205 | ENST00000526103.1 | 753 | No protein | Retained intron | - | - | - | TSL:4, |
| PAK1-214 | ENST00000530358.1 | 735 | No protein | Retained intron | - | - | - | TSL:5, |
| PAK1-219 | ENST00000533468.5 | 503 | No protein | Retained intron | - | - | - | TSL:2, |
| PAK1-206 | ENST00000526910.1 | 348 | No protein | Retained intron | - | - | - | TSL:2, |
| PAK1-216 | ENST00000532711.1 | 308 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PAK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PAK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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