CRISPR-U™ Gene Knockout Cell Line Strategy

PAK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PAK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PAK1
Gene id 5058
Organism Homo sapiens
Official full symbol p21 (RAC1) activated kinase 1
Gene type protein-coding
Also known as IDDMSSD, PAKalpha, alpha-PAK, p65-PAK
Summary This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PAK1-202 ENST00000356341.8 3459 545aa Protein coding CCDS8250 Q13153-1 NM_002576.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PAK1-201 ENST00000278568.8 2543 553aa Protein coding CCDS44687 Q13153-2 - TSL:2, GENCODE basic,
PAK1-215 ENST00000530617.5 3001 522aa Protein coding - B3KNX7 - TSL:2, GENCODE basic,
PAK1-210 ENST00000528203.5 1878 455aa Protein coding - E9PM17 - TSL:2, GENCODE basic,
PAK1-218 ENST00000533285.1 878 244aa Protein coding - H0YCG5 - CDS 5' incomplete, TSL:3,
PAK1-211 ENST00000528592.5 581 89aa Protein coding - E9PMP2 - CDS 3' incomplete, TSL:3,
PAK1-213 ENST00000529248.5 576 106aa Protein coding - E9PKH9 - TSL:4,
PAK1-203 ENST00000524847.5 559 99aa Protein coding - E9PQW5 - CDS 3' incomplete, TSL:5,
PAK1-207 ENST00000526968.1 502 26aa Protein coding - E9PJF8 - CDS 3' incomplete, TSL:4,
PAK1-212 ENST00000528633.1 450 63aa Protein coding - E9PRP6 - CDS 3' incomplete, TSL:3,
PAK1-208 ENST00000527457.5 1047 167aa Nonsense mediated decay - H0YCM0 - CDS 5' incomplete, TSL:3,
PAK1-209 ENST00000527535.5 576 18aa Nonsense mediated decay - H0YF55 - CDS 5' incomplete, TSL:5,
PAK1-204 ENST00000525542.5 863 No protein Processed transcript - - - TSL:2,
PAK1-217 ENST00000532991.5 806 No protein Retained intron - - - TSL:3,
PAK1-220 ENST00000533568.5 762 No protein Retained intron - - - TSL:3,
PAK1-205 ENST00000526103.1 753 No protein Retained intron - - - TSL:4,
PAK1-214 ENST00000530358.1 735 No protein Retained intron - - - TSL:5,
PAK1-219 ENST00000533468.5 503 No protein Retained intron - - - TSL:2,
PAK1-206 ENST00000526910.1 348 No protein Retained intron - - - TSL:2,
PAK1-216 ENST00000532711.1 308 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PAK1
This KO Strategy loading
Red Cotton™ Notes Gene PAK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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