CRISPR-U™ Gene Knockout Cell Line
Strategy
PRDX1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PRDX1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PRDX1 |
| Gene id | 5052 |
| Organism | Homo sapiens |
| Official full symbol | peroxiredoxin 1 |
| Gene type | protein-coding |
| Also known as | MSP23, NKEF-A, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2 |
| Summary | This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PRDX1-204 | ENST00000424390.2 | 1296 | 199aa | Protein coding | CCDS522 | A0A0A0MSI0 A0A384NPQ2 | - | TSL:2, GENCODE basic, APPRIS P1, |
| PRDX1-201 | ENST00000262746.5 | 1234 | 199aa | Protein coding | CCDS522 | A0A384NPQ2 Q06830 | - | TSL:5, GENCODE basic, APPRIS P1, |
| PRDX1-205 | ENST00000447184.6 | 1107 | 199aa | Protein coding | CCDS522 | A0A0A0MSI0 A0A384NPQ2 | - | TSL:5, GENCODE basic, APPRIS P1, |
| PRDX1-206 | ENST00000676549.1 | 1082 | 199aa | Protein coding | CCDS522 | A0A384NPQ2 | - | GENCODE basic, APPRIS P1, |
| PRDX1-202 | ENST00000319248.13 | 945 | 199aa | Protein coding | CCDS522 | A0A384NPQ2 Q06830 | NM_181697.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PRDX1-203 | ENST00000372079.1 | 609 | 97aa | Protein coding | - | A0A0A0MRQ5 | - | TSL:3, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PRDX1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PRDX1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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