CRISPR-U™ Gene Knockout Cell Line Strategy

PRDX1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PRDX1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PRDX1
Gene id 5052
Organism Homo sapiens
Official full symbol peroxiredoxin 1
Gene type protein-coding
Summary This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PRDX1-204 ENST00000424390.2 1296 199aa Protein coding CCDS522 A0A0A0MSI0 A0A384NPQ2 - TSL:2, GENCODE basic, APPRIS P1,
PRDX1-201 ENST00000262746.5 1234 199aa Protein coding CCDS522 A0A384NPQ2 Q06830 - TSL:5, GENCODE basic, APPRIS P1,
PRDX1-205 ENST00000447184.6 1107 199aa Protein coding CCDS522 A0A0A0MSI0 A0A384NPQ2 - TSL:5, GENCODE basic, APPRIS P1,
PRDX1-206 ENST00000676549.1 1082 199aa Protein coding CCDS522 A0A384NPQ2 - GENCODE basic, APPRIS P1,
PRDX1-202 ENST00000319248.13 945 199aa Protein coding CCDS522 A0A384NPQ2 Q06830 NM_181697.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PRDX1-203 ENST00000372079.1 609 97aa Protein coding - A0A0A0MRQ5 - TSL:3, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PRDX1
This KO Strategy loading
Red Cotton™ Notes Gene PRDX1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×