CRISPR-U™ Gene Knockout Cell Line Strategy

PAFAH1B3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PAFAH1B3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PAFAH1B3
Gene id 5050
Organism Homo sapiens
Official full symbol platelet activating factor acetylhydrolase 1b catalytic subunit 3
Gene type protein-coding
Also known as PAFAHG
Summary This gene encodes an acetylhydrolase that catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. The encoded enzyme is a subunit of the platelet-activating factor acetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex functions in brain development. A translocation between this gene on chromosome 19 and the CDC-like kinase 2 gene on chromosome 1 has been observed, and was associated with cognitive disability, ataxia, and atrophy of the brain. Alternatively spliced transcript variants have been described.
Genomic regions Chromosome 19
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PAFAH1B3-202 ENST00000538771.5 1098 231aa Protein coding CCDS12602 A0A024R0L6 Q15102 - TSL:2, GENCODE basic, APPRIS P1,
PAFAH1B3-201 ENST00000262890.8 1054 231aa Protein coding CCDS12602 A0A024R0L6 Q15102 NM_002573.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PAFAH1B3-203 ENST00000594989.5 649 202aa Protein coding - M0R1K3 - CDS 5' incomplete, TSL:5,
PAFAH1B3-208 ENST00000601865.5 646 96aa Protein coding - M0R323 - CDS 3' incomplete, TSL:2,
PAFAH1B3-204 ENST00000595530.5 582 164aa Protein coding - M0R389 - CDS 3' incomplete, TSL:2,
PAFAH1B3-205 ENST00000596265.5 465 139aa Protein coding - M0QZT2 - CDS 3' incomplete, TSL:3,
PAFAH1B3-207 ENST00000599778.1 380 95aa Protein coding - M0QXS6 - CDS 3' incomplete, TSL:5,
PAFAH1B3-206 ENST00000597333.1 726 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PAFAH1B3
This KO Strategy loading
Red Cotton™ Notes Gene PAFAH1B3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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