CRISPR-U™ Gene Knockout Cell Line Strategy

P2RX5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human P2RX5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol P2RX5
Gene id 5026
Organism Homo sapiens
Official full symbol purinergic receptor P2X 5
Gene type protein-coding
Also known as LRH-1, P2X5, P2X5R
Summary The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream gene, TAX1BP3 (Tax1 binding protein 3).
Genomic regions Chromosome 17
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
P2RX5-201 ENST00000225328.10 2060 422aa Protein coding CCDS11034 Q93086-3 NM_002561.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
P2RX5-202 ENST00000345901.7 2031 398aa Protein coding CCDS56014 Q93086-5 - TSL:5, GENCODE basic,
P2RX5-203 ENST00000547178.5 1998 421aa Protein coding CCDS56015 Q93086-1 - TSL:1, GENCODE basic, APPRIS ALT2,
P2RX5-207 ENST00000551178.5 1863 397aa Protein coding CCDS11035 Q93086-2 - TSL:1, GENCODE basic,
P2RX5-209 ENST00000552276.5 1642 431aa Protein coding - K7EQ78 - TSL:5, GENCODE basic, APPRIS ALT2,
P2RX5-208 ENST00000552050.5 1536 362aa Protein coding - B4DEG2 - TSL:2, GENCODE basic,
P2RX5-211 ENST00000552723.1 858 286aa Protein coding - H0YII8 - CDS 5' and 3' incomplete, TSL:5,
P2RX5-210 ENST00000552456.1 851 29aa Nonsense mediated decay - H0YHT9 - CDS 5' incomplete, TSL:5,
P2RX5-206 ENST00000550772.1 429 No protein Processed transcript - - - TSL:3,
P2RX5-205 ENST00000549063.1 1967 No protein Retained intron - - - TSL:2,
P2RX5-204 ENST00000547933.1 1111 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene P2RX5
This KO Strategy loading
Red Cotton™ Notes Gene P2RX5 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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