CRISPR-U™ Gene Knockout Cell Line
Strategy
P2RX5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human P2RX5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | P2RX5 |
| Gene id | 5026 |
| Organism | Homo sapiens |
| Official full symbol | purinergic receptor P2X 5 |
| Gene type | protein-coding |
| Also known as | LRH-1, P2X5, P2X5R |
| Summary | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream gene, TAX1BP3 (Tax1 binding protein 3). |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| P2RX5-201 | ENST00000225328.10 | 2060 | 422aa | Protein coding | CCDS11034 | Q93086-3 | NM_002561.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| P2RX5-202 | ENST00000345901.7 | 2031 | 398aa | Protein coding | CCDS56014 | Q93086-5 | - | TSL:5, GENCODE basic, |
| P2RX5-203 | ENST00000547178.5 | 1998 | 421aa | Protein coding | CCDS56015 | Q93086-1 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| P2RX5-207 | ENST00000551178.5 | 1863 | 397aa | Protein coding | CCDS11035 | Q93086-2 | - | TSL:1, GENCODE basic, |
| P2RX5-209 | ENST00000552276.5 | 1642 | 431aa | Protein coding | - | K7EQ78 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| P2RX5-208 | ENST00000552050.5 | 1536 | 362aa | Protein coding | - | B4DEG2 | - | TSL:2, GENCODE basic, |
| P2RX5-211 | ENST00000552723.1 | 858 | 286aa | Protein coding | - | H0YII8 | - | CDS 5' and 3' incomplete, TSL:5, |
| P2RX5-210 | ENST00000552456.1 | 851 | 29aa | Nonsense mediated decay | - | H0YHT9 | - | CDS 5' incomplete, TSL:5, |
| P2RX5-206 | ENST00000550772.1 | 429 | No protein | Processed transcript | - | - | - | TSL:3, |
| P2RX5-205 | ENST00000549063.1 | 1967 | No protein | Retained intron | - | - | - | TSL:2, |
| P2RX5-204 | ENST00000547933.1 | 1111 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | P2RX5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene P2RX5 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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