CRISPR-U™ Gene Knockout Cell Line
Strategy
OXCT1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human OXCT1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | OXCT1 |
| Gene id | 5019 |
| Organism | Homo sapiens |
| Official full symbol | 3-oxoacid CoA-transferase 1 |
| Gene type | protein-coding |
| Also known as | OXCT, SCOT |
| Summary | This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| OXCT1-201 | ENST00000196371.10 | 3294 | 520aa | Protein coding | CCDS3937 | P55809-1 | NM_000436.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| OXCT1-206 | ENST00000512084.5 | 1593 | 123aa | Protein coding | - | P55809-2 | - | TSL:2, GENCODE basic, |
| OXCT1-204 | ENST00000509987.1 | 1441 | 334aa | Protein coding | - | E9PDW2 | - | TSL:2, GENCODE basic, |
| OXCT1-205 | ENST00000510634.5 | 1441 | 123aa | Protein coding | - | P55809-2 | - | TSL:2, GENCODE basic, |
| OXCT1-207 | ENST00000513081.5 | 350 | No protein | Processed transcript | - | - | - | TSL:5, |
| OXCT1-208 | ENST00000514723.1 | 241 | No protein | Processed transcript | - | - | - | TSL:5, |
| OXCT1-203 | ENST00000508557.5 | 596 | No protein | Retained intron | - | - | - | TSL:2, |
| OXCT1-202 | ENST00000503374.1 | 364 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | OXCT1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene OXCT1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: