CRISPR-U™ Gene Knockout Cell Line Strategy

OXCT1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human OXCT1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol OXCT1
Gene id 5019
Organism Homo sapiens
Official full symbol 3-oxoacid CoA-transferase 1
Gene type protein-coding
Also known as OXCT, SCOT
Summary This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.
Genomic regions Chromosome 5
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
OXCT1-201 ENST00000196371.10 3294 520aa Protein coding CCDS3937 P55809-1 NM_000436.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
OXCT1-206 ENST00000512084.5 1593 123aa Protein coding - P55809-2 - TSL:2, GENCODE basic,
OXCT1-204 ENST00000509987.1 1441 334aa Protein coding - E9PDW2 - TSL:2, GENCODE basic,
OXCT1-205 ENST00000510634.5 1441 123aa Protein coding - P55809-2 - TSL:2, GENCODE basic,
OXCT1-207 ENST00000513081.5 350 No protein Processed transcript - - - TSL:5,
OXCT1-208 ENST00000514723.1 241 No protein Processed transcript - - - TSL:5,
OXCT1-203 ENST00000508557.5 596 No protein Retained intron - - - TSL:2,
OXCT1-202 ENST00000503374.1 364 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene OXCT1
This KO Strategy loading
Red Cotton™ Notes Gene OXCT1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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