CRISPR-U™ Gene Knockout Cell Line Strategy

OXA1L Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human OXA1L Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol OXA1L
Gene id 5018
Organism Homo sapiens
Official full symbol OXA1L mitochondrial inner membrane protein
Gene type protein-coding
Also known as OXA1
Summary This gene encodes an evolutionarily conserved protein that is localized to the inner mitochondrial membrane. The encoded protein is essential for the translocation of the N-terminal tail of subunit 2 of cytochrome c oxidase, and is involved in the assembly of the cytochrome c oxidase and ATPase complexes of the mitochondrial respiratory chain.
Genomic regions Chromosome 14
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
OXA1L-201 ENST00000285848.9 1719 495aa Protein coding CCDS9573 J3KNA0 - TSL:1, GENCODE basic, APPRIS P4,
OXA1L-212 ENST00000612549.6 2806 435aa Protein coding - A0A087X0L7 NM_005015.5 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
OXA1L-202 ENST00000358043.5 1750 419aa Protein coding - Q15070 - TSL:2, GENCODE basic, APPRIS ALT2,
OXA1L-203 ENST00000412791.5 1440 408aa Protein coding - E7EVY0 - TSL:5, GENCODE basic, APPRIS ALT2,
OXA1L-204 ENST00000431881.6 889 112aa Protein coding - C9JC63 - CDS 3' incomplete, TSL:3,
OXA1L-205 ENST00000442110.6 691 78aa Nonsense mediated decay - H7BZE6 - TSL:5,
OXA1L-209 ENST00000495424.5 1679 No protein Retained intron - - - TSL:2,
OXA1L-207 ENST00000481218.1 1596 No protein Retained intron - - - TSL:5,
OXA1L-208 ENST00000483939.6 1224 No protein Retained intron - - - TSL:2,
OXA1L-206 ENST00000473744.5 824 No protein Retained intron - - - TSL:3,
OXA1L-210 ENST00000556473.1 593 No protein Retained intron - - - TSL:4,
OXA1L-211 ENST00000557299.5 538 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene OXA1L
This KO Strategy loading
Red Cotton™ Notes Gene OXA1L had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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