CRISPR-U™ Gene Knockout Cell Line
Strategy
OTX2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human OTX2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | OTX2 |
| Gene id | 5015 |
| Organism | Homo sapiens |
| Official full symbol | orthodenticle homeobox 2 |
| Gene type | protein-coding |
| Also known as | CPHD6, MCOPS5 |
| Summary | This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| OTX2-209 | ENST00000672264.2 | 3011 | 297aa | Protein coding | CCDS9728 | P32243-2 | NM_021728.4 | GENCODE basic, MANE Select v0.92, |
| OTX2-201 | ENST00000339475.10 | 2956 | 289aa | Protein coding | CCDS41960 | P32243-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| OTX2-211 | ENST00000673481.1 | 2240 | 297aa | Protein coding | CCDS9728 | P32243-2 | - | GENCODE basic, |
| OTX2-210 | ENST00000673035.1 | 2154 | 289aa | Protein coding | CCDS41960 | P32243-1 | - | GENCODE basic, APPRIS P1, |
| OTX2-202 | ENST00000408990.8 | 2109 | 289aa | Protein coding | CCDS41960 | P32243-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| OTX2-206 | ENST00000555006.5 | 1279 | 289aa | Protein coding | CCDS41960 | P32243-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| OTX2-208 | ENST00000672125.1 | 1850 | 168aa | Protein coding | - | A0A5F9ZHY1 | - | GENCODE basic, |
| OTX2-205 | ENST00000554845.1 | 908 | 238aa | Protein coding | - | G3V3P9 | - | CDS 3' incomplete, TSL:1, |
| OTX2-204 | ENST00000554788.5 | 835 | 35aa | Protein coding | - | F1T0C9 | - | TSL:1, GENCODE basic, |
| OTX2-207 | ENST00000555804.1 | 797 | 163aa | Protein coding | - | G3V3J3 | - | CDS 3' incomplete, TSL:4, |
| OTX2-203 | ENST00000554559.5 | 715 | 35aa | Protein coding | - | F1T0C9 | - | TSL:5, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | OTX2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene OTX2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: