CRISPR-U™ Gene Knockout Cell Line Strategy

OTX2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human OTX2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol OTX2
Gene id 5015
Organism Homo sapiens
Official full symbol orthodenticle homeobox 2
Gene type protein-coding
Also known as CPHD6, MCOPS5
Summary This gene encodes a member of the bicoid subfamily of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and plays a role in brain, craniofacial, and sensory organ development. The encoded protein also influences the proliferation and differentiation of dopaminergic neuronal progenitor cells during mitosis. Mutations in this gene cause syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). This gene is also suspected of having an oncogenic role in medulloblastoma. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Pseudogenes of this gene are known to exist on chromosomes two and nine.
Genomic regions Chromosome 14
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
OTX2-209 ENST00000672264.2 3011 297aa Protein coding CCDS9728 P32243-2 NM_021728.4 GENCODE basic, MANE Select v0.92,
OTX2-201 ENST00000339475.10 2956 289aa Protein coding CCDS41960 P32243-1 - TSL:1, GENCODE basic, APPRIS P1,
OTX2-211 ENST00000673481.1 2240 297aa Protein coding CCDS9728 P32243-2 - GENCODE basic,
OTX2-210 ENST00000673035.1 2154 289aa Protein coding CCDS41960 P32243-1 - GENCODE basic, APPRIS P1,
OTX2-202 ENST00000408990.8 2109 289aa Protein coding CCDS41960 P32243-1 - TSL:1, GENCODE basic, APPRIS P1,
OTX2-206 ENST00000555006.5 1279 289aa Protein coding CCDS41960 P32243-1 - TSL:1, GENCODE basic, APPRIS P1,
OTX2-208 ENST00000672125.1 1850 168aa Protein coding - A0A5F9ZHY1 - GENCODE basic,
OTX2-205 ENST00000554845.1 908 238aa Protein coding - G3V3P9 - CDS 3' incomplete, TSL:1,
OTX2-204 ENST00000554788.5 835 35aa Protein coding - F1T0C9 - TSL:1, GENCODE basic,
OTX2-207 ENST00000555804.1 797 163aa Protein coding - G3V3J3 - CDS 3' incomplete, TSL:4,
OTX2-203 ENST00000554559.5 715 35aa Protein coding - F1T0C9 - TSL:5, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene OTX2
This KO Strategy loading
Red Cotton™ Notes Gene OTX2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×