CRISPR-U™ Gene Knockout Cell Line Strategy

OAZ1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human OAZ1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol OAZ1
Gene id 4946
Organism Homo sapiens
Official full symbol ornithine decarboxylase antizyme 1
Gene type protein-coding
Also known as AZ1, AZI, OAZ
Summary The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
OAZ1-211 ENST00000602676.6 1148 228aa Protein coding CCDS58639 P54368-1 - TSL:5, GENCODE basic, APPRIS P4,
OAZ1-202 ENST00000582888.8 969 226aa Protein coding CCDS77214 J3QQY4 - TSL:5, GENCODE basic, APPRIS ALT1,
OAZ1-204 ENST00000588673.3 780 208aa Protein coding - K7ERW1 - CDS 3' incomplete, TSL:3,
OAZ1-207 ENST00000590943.6 1012 52aa Nonsense mediated decay - K7EPM6 - TSL:2,
OAZ1-203 ENST00000586054.2 2832 No protein Retained intron - - - TSL:NA,
OAZ1-210 ENST00000593012.1 1357 No protein Retained intron - - - TSL:2,
OAZ1-201 ENST00000581150.6 1131 No protein Retained intron - - - TSL:5,
OAZ1-209 ENST00000592787.2 857 No protein Retained intron - - - TSL:2,
OAZ1-208 ENST00000592727.3 726 No protein Retained intron - - - TSL:2,
OAZ1-205 ENST00000589361.2 679 No protein Retained intron - - - TSL:1,
OAZ1-206 ENST00000589739.3 657 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene OAZ1
This KO Strategy loading
Red Cotton™ Notes Gene OAZ1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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