CRISPR-U™ Gene Knockout Cell Line
Strategy
OAZ1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human OAZ1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | OAZ1 |
| Gene id | 4946 |
| Organism | Homo sapiens |
| Official full symbol | ornithine decarboxylase antizyme 1 |
| Gene type | protein-coding |
| Also known as | AZ1, AZI, OAZ |
| Summary | The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| OAZ1-211 | ENST00000602676.6 | 1148 | 228aa | Protein coding | CCDS58639 | P54368-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| OAZ1-202 | ENST00000582888.8 | 969 | 226aa | Protein coding | CCDS77214 | J3QQY4 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| OAZ1-204 | ENST00000588673.3 | 780 | 208aa | Protein coding | - | K7ERW1 | - | CDS 3' incomplete, TSL:3, |
| OAZ1-207 | ENST00000590943.6 | 1012 | 52aa | Nonsense mediated decay | - | K7EPM6 | - | TSL:2, |
| OAZ1-203 | ENST00000586054.2 | 2832 | No protein | Retained intron | - | - | - | TSL:NA, |
| OAZ1-210 | ENST00000593012.1 | 1357 | No protein | Retained intron | - | - | - | TSL:2, |
| OAZ1-201 | ENST00000581150.6 | 1131 | No protein | Retained intron | - | - | - | TSL:5, |
| OAZ1-209 | ENST00000592787.2 | 857 | No protein | Retained intron | - | - | - | TSL:2, |
| OAZ1-208 | ENST00000592727.3 | 726 | No protein | Retained intron | - | - | - | TSL:2, |
| OAZ1-205 | ENST00000589361.2 | 679 | No protein | Retained intron | - | - | - | TSL:1, |
| OAZ1-206 | ENST00000589739.3 | 657 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | OAZ1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene OAZ1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
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$599
Single gRNA lentivirus
Work flow
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