CRISPR-U™ Gene Knockout Cell Line Strategy

ATP2B4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATP2B4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATP2B4
Gene id 493
Organism Homo sapiens
Official full symbol ATPase plasma membrane Ca2+ transporting 4
Gene type protein-coding
Also known as ATP2B2, MXRA1, PMCA4, PMCA4b, PMCA4x
Summary The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified.
Genomic regions Chromosome 1
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATP2B4-204 ENST00000367218.7 8918 1170aa Protein coding CCDS30977 P23634-2 - TSL:1, GENCODE basic,
ATP2B4-203 ENST00000357681.10 8697 1205aa Protein coding CCDS1440 P23634-6 NM_001684.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ATP2B4-201 ENST00000341360.6 4658 1170aa Protein coding CCDS30977 P23634-2 - TSL:1, GENCODE basic,
ATP2B4-202 ENST00000356729.5 613 129aa Protein coding - H7BY13 - CDS 5' incomplete, TSL:5,
ATP2B4-205 ENST00000458092.5 429 117aa Protein coding - H7BZS8 - CDS 5' incomplete, TSL:3,
ATP2B4-207 ENST00000484746.1 4697 102aa Nonsense mediated decay - H0YDG5 - CDS 5' incomplete, TSL:1,
ATP2B4-206 ENST00000466407.1 388 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATP2B4
This KO Strategy loading
Red Cotton™ Notes Gene ATP2B4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
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