CRISPR-U™ Gene Knockout Cell Line
Strategy
ATP2B4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATP2B4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATP2B4 |
| Gene id | 493 |
| Organism | Homo sapiens |
| Official full symbol | ATPase plasma membrane Ca2+ transporting 4 |
| Gene type | protein-coding |
| Also known as | ATP2B2, MXRA1, PMCA4, PMCA4b, PMCA4x |
| Summary | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATP2B4-204 | ENST00000367218.7 | 8918 | 1170aa | Protein coding | CCDS30977 | P23634-2 | - | TSL:1, GENCODE basic, |
| ATP2B4-203 | ENST00000357681.10 | 8697 | 1205aa | Protein coding | CCDS1440 | P23634-6 | NM_001684.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ATP2B4-201 | ENST00000341360.6 | 4658 | 1170aa | Protein coding | CCDS30977 | P23634-2 | - | TSL:1, GENCODE basic, |
| ATP2B4-202 | ENST00000356729.5 | 613 | 129aa | Protein coding | - | H7BY13 | - | CDS 5' incomplete, TSL:5, |
| ATP2B4-205 | ENST00000458092.5 | 429 | 117aa | Protein coding | - | H7BZS8 | - | CDS 5' incomplete, TSL:3, |
| ATP2B4-207 | ENST00000484746.1 | 4697 | 102aa | Nonsense mediated decay | - | H0YDG5 | - | CDS 5' incomplete, TSL:1, |
| ATP2B4-206 | ENST00000466407.1 | 388 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATP2B4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATP2B4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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