CRISPR-U™ Gene Knockout Cell Line
Strategy
NUCB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NUCB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NUCB1 |
| Gene id | 4924 |
| Organism | Homo sapiens |
| Official full symbol | nucleobindin 1 |
| Gene type | protein-coding |
| Also known as | CALNUC, NUC |
| Summary | This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NUCB1-201 | ENST00000405315.9 | 2406 | 461aa | Protein coding | CCDS12740 | Q02818 | NM_006184.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NUCB1-202 | ENST00000407032.5 | 2158 | 461aa | Protein coding | CCDS12740 | Q02818 | - | TSL:5, GENCODE basic, APPRIS P1, |
| NUCB1-204 | ENST00000424608.1 | 884 | 295aa | Protein coding | - | H7BZI1 | - | CDS 5' and 3' incomplete, TSL:5, |
| NUCB1-207 | ENST00000452087.5 | 864 | 267aa | Protein coding | - | C9JKZ2 | - | CDS 3' incomplete, TSL:3, |
| NUCB1-203 | ENST00000411700.5 | 673 | 124aa | Protein coding | - | C9J3C1 | - | CDS 3' incomplete, TSL:4, |
| NUCB1-206 | ENST00000451312.5 | 563 | 110aa | Protein coding | - | C9JBD3 | - | CDS 3' incomplete, TSL:2, |
| NUCB1-205 | ENST00000443560.1 | 551 | 75aa | Nonsense mediated decay | - | F8WB20 | - | TSL:4, |
| NUCB1-211 | ENST00000485798.5 | 938 | No protein | Processed transcript | - | - | - | TSL:3, |
| NUCB1-212 | ENST00000492367.1 | 1515 | No protein | Retained intron | - | - | - | TSL:2, |
| NUCB1-209 | ENST00000465524.5 | 839 | No protein | Retained intron | - | - | - | TSL:5, |
| NUCB1-210 | ENST00000469291.1 | 582 | No protein | Retained intron | - | - | - | TSL:2, |
| NUCB1-208 | ENST00000460125.1 | 528 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NUCB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NUCB1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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