CRISPR-U™ Gene Knockout Cell Line Strategy

NUCB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NUCB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NUCB1
Gene id 4924
Organism Homo sapiens
Official full symbol nucleobindin 1
Gene type protein-coding
Also known as CALNUC, NUC
Summary This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events.
Genomic regions Chromosome 19
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NUCB1-201 ENST00000405315.9 2406 461aa Protein coding CCDS12740 Q02818 NM_006184.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NUCB1-202 ENST00000407032.5 2158 461aa Protein coding CCDS12740 Q02818 - TSL:5, GENCODE basic, APPRIS P1,
NUCB1-204 ENST00000424608.1 884 295aa Protein coding - H7BZI1 - CDS 5' and 3' incomplete, TSL:5,
NUCB1-207 ENST00000452087.5 864 267aa Protein coding - C9JKZ2 - CDS 3' incomplete, TSL:3,
NUCB1-203 ENST00000411700.5 673 124aa Protein coding - C9J3C1 - CDS 3' incomplete, TSL:4,
NUCB1-206 ENST00000451312.5 563 110aa Protein coding - C9JBD3 - CDS 3' incomplete, TSL:2,
NUCB1-205 ENST00000443560.1 551 75aa Nonsense mediated decay - F8WB20 - TSL:4,
NUCB1-211 ENST00000485798.5 938 No protein Processed transcript - - - TSL:3,
NUCB1-212 ENST00000492367.1 1515 No protein Retained intron - - - TSL:2,
NUCB1-209 ENST00000465524.5 839 No protein Retained intron - - - TSL:5,
NUCB1-210 ENST00000469291.1 582 No protein Retained intron - - - TSL:2,
NUCB1-208 ENST00000460125.1 528 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NUCB1
This KO Strategy loading
Red Cotton™ Notes Gene NUCB1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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