CRISPR-U™ Gene Knockout Cell Line
Strategy
NTRK2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NTRK2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NTRK2 |
| Gene id | 4915 |
| Organism | Homo sapiens |
| Official full symbol | neurotrophic receptor tyrosine kinase 2 |
| Gene type | protein-coding |
| Also known as | EIEE58, GP145-TrkB, OBHD, TRKB, trk-B |
| Summary | This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NTRK2-206 | ENST00000376213.6 | 8693 | 822aa | Protein coding | CCDS35050 | Q16620-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| NTRK2-201 | ENST00000277120.8 | 8666 | 838aa | Protein coding | CCDS6671 | Q16620-4 | NM_006180.6 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| NTRK2-205 | ENST00000376208.6 | 8633 | 537aa | Protein coding | CCDS35052 | Q16620-3 | - | TSL:1, GENCODE basic, |
| NTRK2-203 | ENST00000323115.10 | 8618 | 822aa | Protein coding | CCDS35050 | Q16620-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| NTRK2-202 | ENST00000304053.10 | 8226 | 553aa | Protein coding | CCDS35051 | Q16620-5 | - | TSL:1, GENCODE basic, |
| NTRK2-207 | ENST00000395882.6 | 7499 | 477aa | Protein coding | CCDS35053 | Q16620-2 | - | TSL:1, GENCODE basic, |
| NTRK2-204 | ENST00000359847.4 | 7286 | 477aa | Protein coding | CCDS35053 | Q16620-2 | - | TSL:1, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NTRK2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NTRK2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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