CRISPR-U™ Gene Knockout Cell Line
Strategy
NRAS Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NRAS Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NRAS |
| Gene id | 4893 |
| Organism | Homo sapiens |
| Official full symbol | NRAS proto-oncogene, GTPase |
| Gene type | protein-coding |
| Also known as | ALPS4, CMNS, N-ras, NCMS, NRAS1, NS6 |
| Summary | This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NRAS-201 | ENST00000369535.5 | 4326 | 189aa | Protein coding | CCDS877 | P01111 Q5U091 | NM_002524.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NRAS |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NRAS had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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