CRISPR-U™ Gene Knockout Cell Line Strategy

NPR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NPR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NPR1
Gene id 4881
Organism Homo sapiens
Official full symbol natriuretic peptide receptor 1
Gene type protein-coding
Also known as ANPRA, ANPa, GUC2A, GUCY2A, NPRA
Summary Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 ). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).
Genomic regions Chromosome 1
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NPR1-202 ENST00000368680.4 4185 1061aa Protein coding CCDS1051 A0A140VJE6 P16066 NM_000906.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NPR1-203 ENST00000413826.1 473 No protein Processed transcript - - - TSL:3,
NPR1-201 ENST00000368677.2 1706 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NPR1
This KO Strategy loading
Red Cotton™ Notes Gene NPR1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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