CRISPR-U™ Gene Knockout Cell Line
Strategy
NPPA Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NPPA Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NPPA |
| Gene id | 4878 |
| Organism | Homo sapiens |
| Official full symbol | natriuretic peptide A |
| Gene type | protein-coding |
| Also known as | ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND |
| Summary | The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NPPA-202 | ENST00000376480.7 | 855 | 151aa | Protein coding | CCDS139 | P01160 | NM_006172.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| NPPA-203 | ENST00000610706.1 | 849 | 152aa | Protein coding | - | A0A087X094 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| NPPA-201 | ENST00000376476.1 | 728 | 101aa | Protein coding | - | B0ZBE8 | - | TSL:3, GENCODE basic, APPRIS ALT2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NPPA |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NPPA had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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