CRISPR-U™ Gene Knockout Cell Line Strategy

PNP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PNP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PNP
Gene id 4860
Organism Homo sapiens
Official full symbol purine nucleoside phosphorylase
Gene type protein-coding
Also known as NP, PRO1837, PUNP
Summary This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
Genomic regions Chromosome 14
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PNP-201 ENST00000361505.10 1477 289aa Protein coding CCDS9552 P00491 V9HWH6 NM_000270.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PNP-203 ENST00000553591.1 770 221aa Protein coding - G3V5M2 - CDS 3' incomplete, TSL:5,
PNP-202 ENST00000553418.5 557 93aa Protein coding - G3V2H3 - CDS 3' incomplete, TSL:3,
PNP-205 ENST00000554065.1 554 61aa Protein coding - G3V393 - CDS 3' incomplete, TSL:4,
PNP-207 ENST00000556754.1 2573 No protein Retained intron - - - TSL:2,
PNP-204 ENST00000554056.5 1635 No protein Retained intron - - - TSL:5,
PNP-206 ENST00000556293.5 1023 No protein Retained intron - - - TSL:1,
PNP-208 ENST00000557229.5 992 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PNP
This KO Strategy loading
Red Cotton™ Notes Gene PNP had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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