CRISPR-U™ Gene Knockout Cell Line Strategy

NOTCH2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NOTCH2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NOTCH2
Gene id 4853
Organism Homo sapiens
Official full symbol notch receptor 2
Gene type protein-coding
Also known as AGS2, HJCYS, hN2
Summary This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NOTCH2-201 ENST00000256646.7 11425 2471aa Protein coding CCDS908 Q04721 NM_024408.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NOTCH2-207 ENST00000602566.6 5041 236aa Protein coding - R4GN02 - TSL:1, GENCODE basic,
NOTCH2-206 ENST00000579475.7 2954 863aa Protein coding - D2WEZ3 - TSL:1, GENCODE basic,
NOTCH2-212 ENST00000652302.1 1847 274aa Protein coding - A0A494C1U9 - GENCODE basic,
NOTCH2-211 ENST00000652264.1 593 114aa Protein coding - A0A494C1F1 - CDS 3' incomplete,
NOTCH2-213 ENST00000652737.1 367 12aa Protein coding - A0A494C1H8 - CDS 3' incomplete,
NOTCH2-208 ENST00000640021.1 2058 31aa Nonsense mediated decay - A0A1W2PQQ5 - CDS 5' incomplete, TSL:5,
NOTCH2-210 ENST00000651371.1 492 25aa Nonsense mediated decay - A0A494C049 - CDS 5' incomplete,
NOTCH2-209 ENST00000650638.1 2917 No protein Processed transcript - - - -
NOTCH2-205 ENST00000493703.1 553 No protein Processed transcript - - - TSL:3,
NOTCH2-202 ENST00000478864.1 520 No protein Processed transcript - - - TSL:3,
NOTCH2-204 ENST00000489731.1 414 No protein Processed transcript - - - TSL:5,
NOTCH2-203 ENST00000479412.2 3221 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NOTCH2
This KO Strategy loading
Red Cotton™ Notes Gene NOTCH2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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