CRISPR-U™ Gene Knockout Cell Line
Strategy
NOTCH2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NOTCH2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NOTCH2 |
| Gene id | 4853 |
| Organism | Homo sapiens |
| Official full symbol | notch receptor 2 |
| Gene type | protein-coding |
| Also known as | AGS2, HJCYS, hN2 |
| Summary | This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NOTCH2-201 | ENST00000256646.7 | 11425 | 2471aa | Protein coding | CCDS908 | Q04721 | NM_024408.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NOTCH2-207 | ENST00000602566.6 | 5041 | 236aa | Protein coding | - | R4GN02 | - | TSL:1, GENCODE basic, |
| NOTCH2-206 | ENST00000579475.7 | 2954 | 863aa | Protein coding | - | D2WEZ3 | - | TSL:1, GENCODE basic, |
| NOTCH2-212 | ENST00000652302.1 | 1847 | 274aa | Protein coding | - | A0A494C1U9 | - | GENCODE basic, |
| NOTCH2-211 | ENST00000652264.1 | 593 | 114aa | Protein coding | - | A0A494C1F1 | - | CDS 3' incomplete, |
| NOTCH2-213 | ENST00000652737.1 | 367 | 12aa | Protein coding | - | A0A494C1H8 | - | CDS 3' incomplete, |
| NOTCH2-208 | ENST00000640021.1 | 2058 | 31aa | Nonsense mediated decay | - | A0A1W2PQQ5 | - | CDS 5' incomplete, TSL:5, |
| NOTCH2-210 | ENST00000651371.1 | 492 | 25aa | Nonsense mediated decay | - | A0A494C049 | - | CDS 5' incomplete, |
| NOTCH2-209 | ENST00000650638.1 | 2917 | No protein | Processed transcript | - | - | - | - |
| NOTCH2-205 | ENST00000493703.1 | 553 | No protein | Processed transcript | - | - | - | TSL:3, |
| NOTCH2-202 | ENST00000478864.1 | 520 | No protein | Processed transcript | - | - | - | TSL:3, |
| NOTCH2-204 | ENST00000489731.1 | 414 | No protein | Processed transcript | - | - | - | TSL:5, |
| NOTCH2-203 | ENST00000479412.2 | 3221 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NOTCH2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NOTCH2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Work flow
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