CRISPR-U™ Gene Knockout Cell Line
Strategy
NNMT Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NNMT Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NNMT |
| Gene id | 4837 |
| Organism | Homo sapiens |
| Official full symbol | nicotinamide N-methyltransferase |
| Gene type | protein-coding |
| Summary | N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NNMT-201 | ENST00000299964.4 | 2012 | 264aa | Protein coding | CCDS8368 | P40261 Q6FH49 | NM_006169.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NNMT-203 | ENST00000535401.5 | 1867 | 264aa | Protein coding | CCDS8368 | P40261 Q6FH49 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NNMT-205 | ENST00000541754.1 | 716 | No protein | Processed transcript | - | - | - | TSL:3, |
| NNMT-207 | ENST00000545255.1 | 515 | No protein | Processed transcript | - | - | - | TSL:3, |
| NNMT-206 | ENST00000542647.5 | 511 | No protein | Processed transcript | - | - | - | TSL:2, |
| NNMT-202 | ENST00000535185.5 | 379 | No protein | Processed transcript | - | - | - | TSL:3, |
| NNMT-204 | ENST00000541090.1 | 351 | No protein | Processed transcript | - | - | - | TSL:3, |
| NNMT-208 | ENST00000546313.1 | 665 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NNMT |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NNMT had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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