CRISPR-U™ Gene Knockout Cell Line Strategy

NNMT Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NNMT Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NNMT
Gene id 4837
Organism Homo sapiens
Official full symbol nicotinamide N-methyltransferase
Gene type protein-coding
Summary N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.
Genomic regions Chromosome 11
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NNMT-201 ENST00000299964.4 2012 264aa Protein coding CCDS8368 P40261 Q6FH49 NM_006169.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NNMT-203 ENST00000535401.5 1867 264aa Protein coding CCDS8368 P40261 Q6FH49 - TSL:1, GENCODE basic, APPRIS P1,
NNMT-205 ENST00000541754.1 716 No protein Processed transcript - - - TSL:3,
NNMT-207 ENST00000545255.1 515 No protein Processed transcript - - - TSL:3,
NNMT-206 ENST00000542647.5 511 No protein Processed transcript - - - TSL:2,
NNMT-202 ENST00000535185.5 379 No protein Processed transcript - - - TSL:3,
NNMT-204 ENST00000541090.1 351 No protein Processed transcript - - - TSL:3,
NNMT-208 ENST00000546313.1 665 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NNMT
This KO Strategy loading
Red Cotton™ Notes Gene NNMT had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×