CRISPR-U™ Gene Knockout Cell Line
Strategy
NFKB2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NFKB2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NFKB2 |
| Gene id | 4791 |
| Organism | Homo sapiens |
| Official full symbol | nuclear factor kappa B subunit 2 |
| Gene type | protein-coding |
| Also known as | CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p49/p100, p52 |
| Summary | This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NFKB2-204 | ENST00000428099.6 | 3411 | 899aa | Protein coding | CCDS41565 | Q00653-4 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| NFKB2-203 | ENST00000369966.8 | 3195 | 900aa | Protein coding | CCDS41564 | Q00653-1 | - | TSL:1, GENCODE basic, APPRIS P5, |
| NFKB2-210 | ENST00000652277.1 | 3114 | 899aa | Protein coding | CCDS41565 | Q00653-4 | - | GENCODE basic, APPRIS ALT1, |
| NFKB2-211 | ENST00000661543.1 | 3015 | 900aa | Protein coding | CCDS41564 | Q00653-1 | NM_001322934.2 | GENCODE basic, APPRIS P5, MANE Select v0.92, |
| NFKB2-201 | ENST00000189444.11 | 3012 | 899aa | Protein coding | CCDS41565 | Q00653-4 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| NFKB2-208 | ENST00000601386.5 | 585 | 22aa | Protein coding | - | M0QZX1 | - | CDS 3' incomplete, TSL:4, |
| NFKB2-202 | ENST00000336486.5 | 851 | No protein | Processed transcript | - | - | - | TSL:3, |
| NFKB2-209 | ENST00000651907.1 | 356 | No protein | Processed transcript | - | - | - | - |
| NFKB2-205 | ENST00000467116.5 | 1634 | No protein | Retained intron | - | - | - | TSL:2, |
| NFKB2-206 | ENST00000471698.6 | 801 | No protein | Retained intron | - | - | - | TSL:3, |
| NFKB2-207 | ENST00000473400.1 | 570 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NFKB2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NFKB2 had been KO in hepg2 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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