CRISPR-U™ Gene Knockout Cell Line Strategy

NFKB2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NFKB2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NFKB2
Gene id 4791
Organism Homo sapiens
Official full symbol nuclear factor kappa B subunit 2
Gene type protein-coding
Also known as CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p49/p100, p52
Summary This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 10
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NFKB2-204 ENST00000428099.6 3411 899aa Protein coding CCDS41565 Q00653-4 - TSL:1, GENCODE basic, APPRIS ALT1,
NFKB2-203 ENST00000369966.8 3195 900aa Protein coding CCDS41564 Q00653-1 - TSL:1, GENCODE basic, APPRIS P5,
NFKB2-210 ENST00000652277.1 3114 899aa Protein coding CCDS41565 Q00653-4 - GENCODE basic, APPRIS ALT1,
NFKB2-211 ENST00000661543.1 3015 900aa Protein coding CCDS41564 Q00653-1 NM_001322934.2 GENCODE basic, APPRIS P5, MANE Select v0.92,
NFKB2-201 ENST00000189444.11 3012 899aa Protein coding CCDS41565 Q00653-4 - TSL:1, GENCODE basic, APPRIS ALT1,
NFKB2-208 ENST00000601386.5 585 22aa Protein coding - M0QZX1 - CDS 3' incomplete, TSL:4,
NFKB2-202 ENST00000336486.5 851 No protein Processed transcript - - - TSL:3,
NFKB2-209 ENST00000651907.1 356 No protein Processed transcript - - - -
NFKB2-205 ENST00000467116.5 1634 No protein Retained intron - - - TSL:2,
NFKB2-206 ENST00000471698.6 801 No protein Retained intron - - - TSL:3,
NFKB2-207 ENST00000473400.1 570 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NFKB2
This KO Strategy loading
Red Cotton™ Notes Gene NFKB2 had been KO in hepg2 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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