CRISPR-U™ Gene Knockout Cell Line Strategy

NFE2L1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NFE2L1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NFE2L1
Gene id 4779
Organism Homo sapiens
Official full symbol nuclear factor, erythroid 2 like 1
Gene type protein-coding
Also known as LCR-F1, NRF1, TCF11
Summary This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene, NFE2L1, and for "nuclear respiratory factor 1" which has an official symbol of NRF1.
Genomic regions Chromosome 17
Strategy Summary
This gene has 19 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NFE2L1-203 ENST00000362042.8 4839 772aa Protein coding CCDS11524 Q14494-1 NM_003204.3 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
NFE2L1-202 ENST00000361665.7 4729 761aa Protein coding CCDS82151 J9JIE5 - TSL:5, GENCODE basic,
NFE2L1-201 ENST00000357480.9 4672 742aa Protein coding CCDS82150 Q14494-2 - TSL:1, GENCODE basic, APPRIS P4,
NFE2L1-222 ENST00000585291.5 4390 742aa Protein coding CCDS82150 Q14494-2 - TSL:1, GENCODE basic, APPRIS P4,
NFE2L1-204 ENST00000536222.5 2172 616aa Protein coding - F5H1B7 - TSL:2, GENCODE basic, APPRIS ALT2,
NFE2L1-218 ENST00000583378.5 2051 573aa Protein coding - J3QKY3 - TSL:5, GENCODE basic,
NFE2L1-214 ENST00000582155.5 1998 584aa Protein coding - B4DYE1 - TSL:2, GENCODE basic, APPRIS ALT2,
NFE2L1-220 ENST00000584634.5 588 19aa Protein coding - J3KTG6 - CDS 3' incomplete, TSL:4,
NFE2L1-206 ENST00000577431.1 583 161aa Protein coding - J3QQQ1 - CDS 3' incomplete, TSL:5,
NFE2L1-210 ENST00000580037.1 583 47aa Protein coding - J3QQH8 - CDS 3' incomplete, TSL:4,
NFE2L1-209 ENST00000579889.1 568 9aa Protein coding - A0A0G2JLG9 - CDS 3' incomplete, TSL:3,
NFE2L1-205 ENST00000577411.5 562 62aa Protein coding - J3KSR3 - CDS 3' incomplete, TSL:5,
NFE2L1-221 ENST00000585062.1 556 99aa Protein coding - J3QQY8 - CDS 3' incomplete, TSL:4,
NFE2L1-213 ENST00000581441.1 552 3aa Protein coding - - - CDS 3' incomplete, TSL:4,
NFE2L1-219 ENST00000584137.5 529 82aa Protein coding - J3QLQ1 - CDS 3' incomplete, TSL:4,
NFE2L1-217 ENST00000583210.5 512 23aa Protein coding - J3QR31 - CDS 3' incomplete, TSL:4,
NFE2L1-212 ENST00000581319.1 481 133aa Protein coding - J3KRF3 - CDS 5' incomplete, TSL:3,
NFE2L1-211 ENST00000580050.1 476 111aa Protein coding - J3QRG7 - CDS 3' incomplete, TSL:4,
NFE2L1-208 ENST00000579537.5 372 63aa Protein coding - J3KSE0 - CDS 3' incomplete, TSL:4,
NFE2L1-207 ENST00000579481.1 564 No protein Processed transcript - - - TSL:2,
NFE2L1-215 ENST00000582574.1 556 No protein Processed transcript - - - TSL:3,
NFE2L1-223 ENST00000585299.1 537 No protein Processed transcript - - - TSL:4,
NFE2L1-216 ENST00000583060.1 477 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NFE2L1
This KO Strategy loading
Red Cotton™ Notes Gene NFE2L1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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