CRISPR-U™ Gene Knockout Cell Line Strategy

NFATC3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NFATC3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NFATC3
Gene id 4775
Organism Homo sapiens
Official full symbol nuclear factor of activated T cells 3
Gene type protein-coding
Also known as NF-AT4c, NFAT4, NFATX
Summary The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene.
Genomic regions Chromosome 16
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NFATC3-202 ENST00000346183.8 6328 1075aa Protein coding CCDS10860 Q12968-1 NM_173165.3 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
NFATC3-203 ENST00000349223.9 6328 1065aa Protein coding CCDS10861 Q12968-3 - TSL:1, GENCODE basic,
NFATC3-201 ENST00000329524.8 6144 1068aa Protein coding CCDS10862 Q12968-2 - TSL:1, GENCODE basic, APPRIS P4,
NFATC3-222 ENST00000575270.5 3824 1047aa Protein coding - I3L3K7 - TSL:5, GENCODE basic,
NFATC3-211 ENST00000562926.5 3748 1053aa Protein coding - H3BR83 - CDS 5' incomplete, TSL:1,
NFATC3-221 ENST00000570212.5 3181 1060aa Protein coding - H3BRS1 - CDS 5' incomplete, TSL:1,
NFATC3-215 ENST00000565750.2 529 167aa Protein coding - H3BU30 - CDS 5' incomplete, TSL:3,
NFATC3-206 ENST00000539828.6 2484 38aa Nonsense mediated decay - F8VPH9 - TSL:2,
NFATC3-209 ENST00000561714.5 792 38aa Nonsense mediated decay - F8VPH9 - TSL:3,
NFATC3-219 ENST00000568466.6 737 38aa Nonsense mediated decay - F8VPH9 - TSL:2,
NFATC3-210 ENST00000562171.1 707 41aa Nonsense mediated decay - H3BRM3 - TSL:3,
NFATC3-205 ENST00000535127.2 4194 No protein Processed transcript - - - TSL:1,
NFATC3-213 ENST00000563319.5 4122 No protein Processed transcript - - - TSL:1,
NFATC3-216 ENST00000566301.5 3840 No protein Processed transcript - - - TSL:1,
NFATC3-212 ENST00000563288.5 3790 No protein Processed transcript - - - TSL:1,
NFATC3-214 ENST00000563796.5 3753 No protein Processed transcript - - - TSL:1,
NFATC3-220 ENST00000569766.5 3555 No protein Processed transcript - - - TSL:1,
NFATC3-218 ENST00000567152.5 3186 No protein Processed transcript - - - TSL:1,
NFATC3-217 ENST00000566893.5 715 No protein Processed transcript - - - TSL:2,
NFATC3-208 ENST00000553077.5 3870 No protein Retained intron - - - TSL:2,
NFATC3-207 ENST00000549350.5 3588 No protein Retained intron - - - TSL:2,
NFATC3-204 ENST00000379165.8 3583 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NFATC3
This KO Strategy loading
Red Cotton™ Notes Gene NFATC3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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