CRISPR-U™ Gene Knockout Cell Line Strategy

NFATC1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NFATC1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NFATC1
Gene id 4772
Organism Homo sapiens
Official full symbol nuclear factor of activated T cells 1
Gene type protein-coding
Also known as NF-ATC, NF-ATc1.2, NFAT2, NFATc
Summary The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
Genomic regions Chromosome 18
Strategy Summary
This gene has 11 protein coding transcripts：
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NFATC1-205 ENST00000427363.7 4873 943aa Protein coding CCDS62467 O95644-1 NM_001278669.2 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
NFATC1-217 ENST00000591814.5 4819 716aa Protein coding CCDS59326 O95644-2 - TSL:1, GENCODE basic, APPRIS ALT2,
NFATC1-201 ENST00000253506.9 4642 825aa Protein coding CCDS12015 O95644-4 - TSL:1, GENCODE basic, APPRIS ALT2,
NFATC1-203 ENST00000329101.8 4595 930aa Protein coding CCDS32850 O95644-6 - TSL:1, GENCODE basic, APPRIS ALT2,
NFATC1-202 ENST00000318065.9 4302 812aa Protein coding CCDS59327 O95644-5 - TSL:1, GENCODE basic, APPRIS ALT2,
NFATC1-207 ENST00000545796.5 3558 471aa Protein coding CCDS62471 F5H4S8 - TSL:2, GENCODE basic,
NFATC1-204 ENST00000397790.6 3453 353aa Protein coding CCDS12016 O95644-17 - TSL:1, GENCODE basic,
NFATC1-218 ENST00000592223.5 2531 703aa Protein coding CCDS62470 O95644-3 - TSL:1, GENCODE basic, APPRIS ALT2,
NFATC1-206 ENST00000542384.5 2142 713aa Protein coding CCDS62468 O95644-10 - TSL:1, GENCODE basic, APPRIS ALT2,
NFATC1-208 ENST00000586434.1 2103 700aa Protein coding CCDS62469 O95644-11 - TSL:1, GENCODE basic, APPRIS ALT2,
NFATC1-210 ENST00000587635.5 2195 639aa Protein coding - K7ER53 - TSL:1, GENCODE basic,
NFATC1-213 ENST00000590313.5 1919 71aa Nonsense mediated decay - K7EQ04 - TSL:2,
NFATC1-211 ENST00000590172.1 1668 No protein Processed transcript - - - TSL:3,
NFATC1-209 ENST00000586695.1 1335 No protein Processed transcript - - - TSL:1,
NFATC1-214 ENST00000590861.1 566 No protein Processed transcript - - - TSL:3,
NFATC1-212 ENST00000590224.5 450 No protein Processed transcript - - - TSL:3,
NFATC1-215 ENST00000591065.1 528 No protein Retained intron - - - TSL:2,
NFATC1-216 ENST00000591089.1 504 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NFATC1
This KO Strategy loading
Red Cotton™ Notes Gene NFATC1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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