CRISPR-U™ Gene Knockout Cell Line
Strategy
NEFM Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NEFM Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NEFM |
| Gene id | 4741 |
| Organism | Homo sapiens |
| Official full symbol | neurofilament medium |
| Gene type | protein-coding |
| Also known as | NEF3, NF-M, NFM |
| Summary | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NEFM-201 | ENST00000221166.10 | 3271 | 916aa | Protein coding | CCDS6046 | P07197-1 | NM_005382.2 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| NEFM-202 | ENST00000433454.3 | 2519 | 540aa | Protein coding | CCDS47831 | P07197-2 | - | TSL:2, GENCODE basic, |
| NEFM-203 | ENST00000437366.2 | 3148 | 877aa | Protein coding | - | E7ESP9 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| NEFM-204 | ENST00000518131.5 | 2500 | 698aa | Protein coding | - | E7EMV2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| NEFM-205 | ENST00000521540.1 | 1885 | No protein | Processed transcript | - | - | - | TSL:5, |
| NEFM-206 | ENST00000523467.1 | 582 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NEFM |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NEFM had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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