CRISPR-U™ Gene Knockout Cell Line Strategy

NEFM Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NEFM Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NEFM
Gene id 4741
Organism Homo sapiens
Official full symbol neurofilament medium
Gene type protein-coding
Also known as NEF3, NF-M, NFM
Summary Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Genomic regions Chromosome 8
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NEFM-201 ENST00000221166.10 3271 916aa Protein coding CCDS6046 P07197-1 NM_005382.2 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
NEFM-202 ENST00000433454.3 2519 540aa Protein coding CCDS47831 P07197-2 - TSL:2, GENCODE basic,
NEFM-203 ENST00000437366.2 3148 877aa Protein coding - E7ESP9 - TSL:2, GENCODE basic, APPRIS ALT2,
NEFM-204 ENST00000518131.5 2500 698aa Protein coding - E7EMV2 - TSL:2, GENCODE basic, APPRIS ALT2,
NEFM-205 ENST00000521540.1 1885 No protein Processed transcript - - - TSL:5,
NEFM-206 ENST00000523467.1 582 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NEFM
This KO Strategy loading
Red Cotton™ Notes Gene NEFM had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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