CRISPR-U™ Gene Knockout Cell Line
Strategy
NDUFC1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NDUFC1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NDUFC1 |
| Gene id | 4717 |
| Organism | Homo sapiens |
| Official full symbol | NADH:ubiquinone oxidoreductase subunit C1 |
| Gene type | protein-coding |
| Also known as | KFYI |
| Summary | The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NDUFC1-211 | ENST00000544855.5 | 1257 | 76aa | Protein coding | CCDS3746 | O43677 | - | TSL:3, GENCODE basic, APPRIS P1, |
| NDUFC1-209 | ENST00000539002.5 | 1198 | 76aa | Protein coding | CCDS3746 | O43677 | - | TSL:2, GENCODE basic, APPRIS P1, |
| NDUFC1-206 | ENST00000505036.5 | 809 | 76aa | Protein coding | CCDS3746 | O43677 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NDUFC1-203 | ENST00000394228.5 | 761 | 76aa | Protein coding | CCDS3746 | O43677 | - | TSL:2, GENCODE basic, APPRIS P1, |
| NDUFC1-210 | ENST00000539387.5 | 725 | 76aa | Protein coding | CCDS3746 | O43677 | - | TSL:2, GENCODE basic, APPRIS P1, |
| NDUFC1-201 | ENST00000394223.2 | 696 | 76aa | Protein coding | CCDS3746 | O43677 | NM_001184989.2 | TSL:3, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NDUFC1-212 | ENST00000676245.1 | 2282 | 61aa | Protein coding | - | - | - | GENCODE basic, |
| NDUFC1-205 | ENST00000503997.5 | 628 | 76aa | Nonsense mediated decay | CCDS3746 | O43677 | - | TSL:3, |
| NDUFC1-207 | ENST00000507764.1 | 838 | No protein | Processed transcript | - | - | - | TSL:2, |
| NDUFC1-204 | ENST00000503453.5 | 682 | No protein | Processed transcript | - | - | - | TSL:3, |
| NDUFC1-202 | ENST00000394225.6 | 664 | No protein | Processed transcript | - | - | - | TSL:2, |
| NDUFC1-208 | ENST00000513022.5 | 564 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NDUFC1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NDUFC1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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