CRISPR-U™ Gene Knockout Cell Line Strategy

NDUFB9 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NDUFB9 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NDUFB9
Gene id 4715
Organism Homo sapiens
Official full symbol NADH:ubiquinone oxidoreductase subunit B9
Gene type protein-coding
Also known as B22, CI-B22, LYRM3, MC1DN24, UQOR22
Summary The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 8
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NDUFB9-201 ENST00000276689.8 691 179aa Protein coding CCDS6352 Q9Y6M9 NM_005005.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NDUFB9-202 ENST00000517367.1 636 168aa Protein coding CCDS83324 E9PH64 - TSL:3, GENCODE basic,
NDUFB9-208 ENST00000606244.2 1565 138aa Protein coding - A0A3B3IT57 - TSL:NA, GENCODE basic,
NDUFB9-204 ENST00000518008.5 789 178aa Protein coding - E7EWZ0 - TSL:2, GENCODE basic,
NDUFB9-210 ENST00000677021.1 3541 179aa Nonsense mediated decay CCDS6352 - - -
NDUFB9-213 ENST00000677822.1 2865 162aa Nonsense mediated decay - - - -
NDUFB9-206 ENST00000522532.6 2794 221aa Nonsense mediated decay - E9PF49 - TSL:2,
NDUFB9-209 ENST00000676713.1 2743 47aa Nonsense mediated decay - - - -
NDUFB9-214 ENST00000677950.1 1423 47aa Nonsense mediated decay - - - -
NDUFB9-211 ENST00000677095.1 3828 No protein Processed transcript - - - -
NDUFB9-215 ENST00000678753.1 1673 No protein Processed transcript - - - -
NDUFB9-203 ENST00000517830.1 320 No protein Processed transcript - - - TSL:3,
NDUFB9-207 ENST00000524241.2 4555 No protein Retained intron - - - TSL:2,
NDUFB9-216 ENST00000678801.1 3429 No protein Retained intron - - - -
NDUFB9-212 ENST00000677782.1 3288 No protein Retained intron - - - -
NDUFB9-205 ENST00000518657.6 2496 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NDUFB9
This KO Strategy loading
Red Cotton™ Notes Gene NDUFB9 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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