CRISPR-U™ Gene Knockout Cell Line Strategy

NDUFA6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NDUFA6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NDUFA6
Gene id 4700
Organism Homo sapiens
Official full symbol NADH:ubiquinone oxidoreductase subunit A6
Gene type protein-coding
Also known as B14, CI-B14, LYRM6, MC1DN33, NADHB14
Summary This gene encodes a member of the LYR family of proteins that contain a highly conserved tripeptide (LYR) motif near the N-terminus. The encoded protein is an accessory subunit of NADH: ubiquinone oxidorerductase (Complex I), which is the largest enzyme of the mitochondrial membrane respiratory chain. Complex I functions in electron transfer from NADH to the respiratory chain.
Genomic regions Chromosome 22
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NDUFA6-203 ENST00000617763.1 1183 154aa Protein coding CCDS33656 A0A2Y9D025 - TSL:1, GENCODE basic,
NDUFA6-202 ENST00000498737.8 1072 128aa Protein coding - P56556 NM_002490.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NDUFA6-201 ENST00000470753.1 475 71aa Protein coding - R4GN43 - TSL:2, GENCODE basic,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NDUFA6
This KO Strategy loading
Red Cotton™ Notes Gene NDUFA6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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