CRISPR-U™ Gene Knockout Cell Line
Strategy
NDUFA6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NDUFA6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NDUFA6 |
| Gene id | 4700 |
| Organism | Homo sapiens |
| Official full symbol | NADH:ubiquinone oxidoreductase subunit A6 |
| Gene type | protein-coding |
| Also known as | B14, CI-B14, LYRM6, MC1DN33, NADHB14 |
| Summary | This gene encodes a member of the LYR family of proteins that contain a highly conserved tripeptide (LYR) motif near the N-terminus. The encoded protein is an accessory subunit of NADH: ubiquinone oxidorerductase (Complex I), which is the largest enzyme of the mitochondrial membrane respiratory chain. Complex I functions in electron transfer from NADH to the respiratory chain. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NDUFA6-203 | ENST00000617763.1 | 1183 | 154aa | Protein coding | CCDS33656 | A0A2Y9D025 | - | TSL:1, GENCODE basic, |
| NDUFA6-202 | ENST00000498737.8 | 1072 | 128aa | Protein coding | - | P56556 | NM_002490.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NDUFA6-201 | ENST00000470753.1 | 475 | 71aa | Protein coding | - | R4GN43 | - | TSL:2, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NDUFA6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NDUFA6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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