CRISPR-U™ Gene Knockout Cell Line Strategy

NDUFA2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NDUFA2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NDUFA2
Gene id 4695
Organism Homo sapiens
Official full symbol NADH:ubiquinone oxidoreductase subunit A2
Gene type protein-coding
Also known as B8, CD14, CIB8, MC1DN13
Summary The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 5
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NDUFA2-201 ENST00000252102.9 649 99aa Protein coding CCDS4234 O43678-1 NM_002488.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NDUFA2-204 ENST00000512088.1 627 76aa Protein coding CCDS54911 O43678-2 - TSL:2, GENCODE basic,
NDUFA2-203 ENST00000510680.1 576 No protein Processed transcript - - - TSL:2,
NDUFA2-202 ENST00000502960.1 721 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NDUFA2
This KO Strategy loading
Red Cotton™ Notes Gene NDUFA2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×