CRISPR-U™ Gene Knockout Cell Line
Strategy
NDUFA2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NDUFA2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NDUFA2 |
| Gene id | 4695 |
| Organism | Homo sapiens |
| Official full symbol | NADH:ubiquinone oxidoreductase subunit A2 |
| Gene type | protein-coding |
| Also known as | B8, CD14, CIB8, MC1DN13 |
| Summary | The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NDUFA2-201 | ENST00000252102.9 | 649 | 99aa | Protein coding | CCDS4234 | O43678-1 | NM_002488.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NDUFA2-204 | ENST00000512088.1 | 627 | 76aa | Protein coding | CCDS54911 | O43678-2 | - | TSL:2, GENCODE basic, |
| NDUFA2-203 | ENST00000510680.1 | 576 | No protein | Processed transcript | - | - | - | TSL:2, |
| NDUFA2-202 | ENST00000502960.1 | 721 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NDUFA2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NDUFA2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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