CRISPR-U™ Gene Knockout Cell Line
Strategy
NCK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NCK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NCK1 |
| Gene id | 4690 |
| Organism | Homo sapiens |
| Official full symbol | NCK adaptor protein 1 |
| Gene type | protein-coding |
| Also known as | NCK, NCKalpha, nck-1 |
| Summary | The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NCK1-207 | ENST00000481752.6 | 4451 | 377aa | Protein coding | CCDS3092 | P16333-1 | NM_001291999.2 | TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NCK1-204 | ENST00000469404.1 | 2403 | 313aa | Protein coding | CCDS54644 | P16333-2 | - | TSL:2, GENCODE basic, |
| NCK1-201 | ENST00000288986.6 | 1937 | 377aa | Protein coding | CCDS3092 | P16333-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NCK1-211 | ENST00000491539.5 | 587 | 129aa | Protein coding | - | C9JVV5 | - | CDS 3' incomplete, TSL:4, |
| NCK1-203 | ENST00000467911.1 | 581 | 174aa | Protein coding | - | C9JAB9 | - | CDS 3' incomplete, TSL:3, |
| NCK1-209 | ENST00000485096.5 | 571 | 115aa | Protein coding | - | C9K098 | - | CDS 3' incomplete, TSL:4, |
| NCK1-205 | ENST00000476286.1 | 557 | 75aa | Protein coding | - | C9J0K5 | - | CDS 3' incomplete, TSL:4, |
| NCK1-210 | ENST00000488930.5 | 526 | 162aa | Protein coding | - | C9J869 | - | CDS 3' incomplete, TSL:5, |
| NCK1-212 | ENST00000496489.5 | 422 | 141aa | Protein coding | - | H7C5C7 | - | CDS 5' and 3' incomplete, TSL:3, |
| NCK1-206 | ENST00000478862.1 | 794 | No protein | Retained intron | - | - | - | TSL:2, |
| NCK1-208 | ENST00000482071.1 | 690 | No protein | Retained intron | - | - | - | TSL:3, |
| NCK1-202 | ENST00000460960.1 | 577 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NCK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NCK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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