CRISPR-U™ Gene Knockout Cell Line Strategy

NCK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NCK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NCK1
Gene id 4690
Organism Homo sapiens
Official full symbol NCK adaptor protein 1
Gene type protein-coding
Also known as NCK, NCKalpha, nck-1
Summary The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Genomic regions Chromosome 3
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NCK1-207 ENST00000481752.6 4451 377aa Protein coding CCDS3092 P16333-1 NM_001291999.2 TSL:5, GENCODE basic, APPRIS P1, MANE Select v0.92,
NCK1-204 ENST00000469404.1 2403 313aa Protein coding CCDS54644 P16333-2 - TSL:2, GENCODE basic,
NCK1-201 ENST00000288986.6 1937 377aa Protein coding CCDS3092 P16333-1 - TSL:1, GENCODE basic, APPRIS P1,
NCK1-211 ENST00000491539.5 587 129aa Protein coding - C9JVV5 - CDS 3' incomplete, TSL:4,
NCK1-203 ENST00000467911.1 581 174aa Protein coding - C9JAB9 - CDS 3' incomplete, TSL:3,
NCK1-209 ENST00000485096.5 571 115aa Protein coding - C9K098 - CDS 3' incomplete, TSL:4,
NCK1-205 ENST00000476286.1 557 75aa Protein coding - C9J0K5 - CDS 3' incomplete, TSL:4,
NCK1-210 ENST00000488930.5 526 162aa Protein coding - C9J869 - CDS 3' incomplete, TSL:5,
NCK1-212 ENST00000496489.5 422 141aa Protein coding - H7C5C7 - CDS 5' and 3' incomplete, TSL:3,
NCK1-206 ENST00000478862.1 794 No protein Retained intron - - - TSL:2,
NCK1-208 ENST00000482071.1 690 No protein Retained intron - - - TSL:3,
NCK1-202 ENST00000460960.1 577 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NCK1
This KO Strategy loading
Red Cotton™ Notes Gene NCK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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