CRISPR-U™ Gene Knockout Cell Line Strategy

NCAM1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NCAM1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NCAM1
Gene id 4684
Organism Homo sapiens
Official full symbol neural cell adhesion molecule 1
Gene type protein-coding
Also known as CD56, MSK39, NCAM
Summary This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 11
Strategy Summary
This gene has 19 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NCAM1-201 ENST00000316851.12 5819 858aa Protein coding CCDS73385 P13591-2 NM_181351.5 TSL:5, GENCODE basic, APPRIS P3, MANE Select v0.92,
NCAM1-228 ENST00000621128.4 4698 726aa Protein coding CCDS73387 P13591-4 - TSL:5, GENCODE basic,
NCAM1-226 ENST00000619839.4 2993 884aa Protein coding CCDS73384 A0A087WWD4 - TSL:5, GENCODE basic,
NCAM1-213 ENST00000531044.5 2826 848aa Protein coding CCDS73388 P13591-1 - TSL:5, GENCODE basic, APPRIS ALT1,
NCAM1-230 ENST00000621850.4 2787 761aa Protein coding CCDS73386 P13591-3 - TSL:5, GENCODE basic,
NCAM1-222 ENST00000615285.4 6045 884aa Protein coding - A0A087WV75 - TSL:5, GENCODE basic,
NCAM1-221 ENST00000615112.4 5967 858aa Protein coding - A0A087WX77 - TSL:5, GENCODE basic, APPRIS ALT1,
NCAM1-225 ENST00000618266.4 5937 848aa Protein coding - A0A087WTF6 - TSL:5, GENCODE basic, APPRIS ALT1,
NCAM1-229 ENST00000621518.4 4922 761aa Protein coding - A0A087WTE4 - TSL:5, GENCODE basic,
NCAM1-202 ENST00000401611.6 2510 725aa Protein coding - H7BYX6 - TSL:5, GENCODE basic,
NCAM1-210 ENST00000529356.5 1843 364aa Protein coding - P13591-6 - TSL:1, GENCODE basic,
NCAM1-208 ENST00000528158.2 1758 468aa Protein coding - A0A0D9SF30 - CDS 5' incomplete, TSL:5,
NCAM1-215 ENST00000533073.5 1648 357aa Protein coding - A0A0D9SF98 - CDS 5' incomplete, TSL:1,
NCAM1-207 ENST00000526322.5 1260 305aa Protein coding - A0A087WVU1 - CDS 5' incomplete, TSL:2,
NCAM1-209 ENST00000528590.5 1179 372aa Protein coding - A0A087WWJ5 - CDS 5' incomplete, TSL:5,
NCAM1-212 ENST00000530543.5 686 228aa Protein coding - A0A087X1V2 - CDS 5' and 3' incomplete, TSL:5,
NCAM1-220 ENST00000613217.4 610 133aa Protein coding - A0A087WVD0 - CDS 3' incomplete, TSL:3,
NCAM1-227 ENST00000620046.4 585 135aa Protein coding - A0A087WZS4 - CDS 3' incomplete, TSL:4,
NCAM1-203 ENST00000524916.1 502 84aa Protein coding - A0A087WTR3 - CDS 5' incomplete, TSL:5,
NCAM1-214 ENST00000531817.5 1423 165aa Nonsense mediated decay - A0A0C4DGS4 - CDS 5' incomplete, TSL:5,
NCAM1-219 ENST00000611284.4 3309 No protein Processed transcript - - - TSL:5,
NCAM1-223 ENST00000615525.1 729 No protein Processed transcript - - - TSL:5,
NCAM1-206 ENST00000525973.5 558 No protein Processed transcript - - - TSL:4,
NCAM1-224 ENST00000617166.1 531 No protein Processed transcript - - - TSL:4,
NCAM1-217 ENST00000534046.2 632 No protein Retained intron - - - TSL:5,
NCAM1-205 ENST00000525691.5 585 No protein Retained intron - - - TSL:5,
NCAM1-211 ENST00000530090.1 565 No protein Retained intron - - - TSL:2,
NCAM1-216 ENST00000533226.2 560 No protein Retained intron - - - TSL:2,
NCAM1-218 ENST00000534516.2 560 No protein Retained intron - - - TSL:3,
NCAM1-204 ENST00000525355.5 559 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NCAM1
This KO Strategy loading
Red Cotton™ Notes Gene NCAM1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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