CRISPR-U™ Gene Knockout Cell Line
Strategy
NCAM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NCAM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NCAM1 |
| Gene id | 4684 |
| Organism | Homo sapiens |
| Official full symbol | neural cell adhesion molecule 1 |
| Gene type | protein-coding |
| Also known as | CD56, MSK39, NCAM |
| Summary | This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 19 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NCAM1-201 | ENST00000316851.12 | 5819 | 858aa | Protein coding | CCDS73385 | P13591-2 | NM_181351.5 | TSL:5, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| NCAM1-228 | ENST00000621128.4 | 4698 | 726aa | Protein coding | CCDS73387 | P13591-4 | - | TSL:5, GENCODE basic, |
| NCAM1-226 | ENST00000619839.4 | 2993 | 884aa | Protein coding | CCDS73384 | A0A087WWD4 | - | TSL:5, GENCODE basic, |
| NCAM1-213 | ENST00000531044.5 | 2826 | 848aa | Protein coding | CCDS73388 | P13591-1 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| NCAM1-230 | ENST00000621850.4 | 2787 | 761aa | Protein coding | CCDS73386 | P13591-3 | - | TSL:5, GENCODE basic, |
| NCAM1-222 | ENST00000615285.4 | 6045 | 884aa | Protein coding | - | A0A087WV75 | - | TSL:5, GENCODE basic, |
| NCAM1-221 | ENST00000615112.4 | 5967 | 858aa | Protein coding | - | A0A087WX77 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| NCAM1-225 | ENST00000618266.4 | 5937 | 848aa | Protein coding | - | A0A087WTF6 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| NCAM1-229 | ENST00000621518.4 | 4922 | 761aa | Protein coding | - | A0A087WTE4 | - | TSL:5, GENCODE basic, |
| NCAM1-202 | ENST00000401611.6 | 2510 | 725aa | Protein coding | - | H7BYX6 | - | TSL:5, GENCODE basic, |
| NCAM1-210 | ENST00000529356.5 | 1843 | 364aa | Protein coding | - | P13591-6 | - | TSL:1, GENCODE basic, |
| NCAM1-208 | ENST00000528158.2 | 1758 | 468aa | Protein coding | - | A0A0D9SF30 | - | CDS 5' incomplete, TSL:5, |
| NCAM1-215 | ENST00000533073.5 | 1648 | 357aa | Protein coding | - | A0A0D9SF98 | - | CDS 5' incomplete, TSL:1, |
| NCAM1-207 | ENST00000526322.5 | 1260 | 305aa | Protein coding | - | A0A087WVU1 | - | CDS 5' incomplete, TSL:2, |
| NCAM1-209 | ENST00000528590.5 | 1179 | 372aa | Protein coding | - | A0A087WWJ5 | - | CDS 5' incomplete, TSL:5, |
| NCAM1-212 | ENST00000530543.5 | 686 | 228aa | Protein coding | - | A0A087X1V2 | - | CDS 5' and 3' incomplete, TSL:5, |
| NCAM1-220 | ENST00000613217.4 | 610 | 133aa | Protein coding | - | A0A087WVD0 | - | CDS 3' incomplete, TSL:3, |
| NCAM1-227 | ENST00000620046.4 | 585 | 135aa | Protein coding | - | A0A087WZS4 | - | CDS 3' incomplete, TSL:4, |
| NCAM1-203 | ENST00000524916.1 | 502 | 84aa | Protein coding | - | A0A087WTR3 | - | CDS 5' incomplete, TSL:5, |
| NCAM1-214 | ENST00000531817.5 | 1423 | 165aa | Nonsense mediated decay | - | A0A0C4DGS4 | - | CDS 5' incomplete, TSL:5, |
| NCAM1-219 | ENST00000611284.4 | 3309 | No protein | Processed transcript | - | - | - | TSL:5, |
| NCAM1-223 | ENST00000615525.1 | 729 | No protein | Processed transcript | - | - | - | TSL:5, |
| NCAM1-206 | ENST00000525973.5 | 558 | No protein | Processed transcript | - | - | - | TSL:4, |
| NCAM1-224 | ENST00000617166.1 | 531 | No protein | Processed transcript | - | - | - | TSL:4, |
| NCAM1-217 | ENST00000534046.2 | 632 | No protein | Retained intron | - | - | - | TSL:5, |
| NCAM1-205 | ENST00000525691.5 | 585 | No protein | Retained intron | - | - | - | TSL:5, |
| NCAM1-211 | ENST00000530090.1 | 565 | No protein | Retained intron | - | - | - | TSL:2, |
| NCAM1-216 | ENST00000533226.2 | 560 | No protein | Retained intron | - | - | - | TSL:2, |
| NCAM1-218 | ENST00000534516.2 | 560 | No protein | Retained intron | - | - | - | TSL:3, |
| NCAM1-204 | ENST00000525355.5 | 559 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NCAM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NCAM1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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Single gRNA lentivirus
Work flow
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