CRISPR-U™ Gene Knockout Cell Line Strategy

NBN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NBN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NBN
Gene id 4683
Organism Homo sapiens
Official full symbol nibrin
Gene type protein-coding
Also known as AT-V1, AT-V2, ATV, NBS, NBS1, P95
Summary Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
Genomic regions Chromosome 8
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NBN-201 ENST00000265433.8 4622 754aa Protein coding CCDS6249 O60934 NM_002485.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NBN-203 ENST00000409330.5 4523 672aa Protein coding CCDS43753 A0A0C4DG07 - TSL:5, GENCODE basic,
NBN-211 ENST00000613033.1 786 124aa Protein coding - A0A087X1V5 - CDS 5' incomplete, TSL:3,
NBN-207 ENST00000517772.5 701 164aa Protein coding - E5RGR7 - CDS 3' incomplete, TSL:3,
NBN-206 ENST00000517337.1 565 92aa Protein coding - E5RGN7 - CDS 3' incomplete, TSL:4,
NBN-208 ENST00000519426.5 535 159aa Protein coding - E5RGU1 - CDS 3' incomplete, TSL:4,
NBN-202 ENST00000396252.6 4907 58aa Nonsense mediated decay - E2QRP0 - TSL:5,
NBN-210 ENST00000523444.1 593 58aa Nonsense mediated decay - E2QRP0 - TSL:4,
NBN-205 ENST00000494804.1 593 No protein Retained intron - - - TSL:3,
NBN-204 ENST00000474821.1 503 No protein Retained intron - - - TSL:2,
NBN-209 ENST00000520325.1 494 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NBN
This KO Strategy loading
Red Cotton™ Notes Gene NBN had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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