CRISPR-U™ Gene Knockout Cell Line
Strategy
NASP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NASP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NASP |
| Gene id | 4678 |
| Organism | Homo sapiens |
| Official full symbol | nuclear autoantigenic sperm protein |
| Gene type | protein-coding |
| Also known as | FLB7527, HMDRA1, PRO1999 |
| Summary | This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NASP-201 | ENST00000350030.8 | 3225 | 788aa | Protein coding | CCDS524 | P49321-1 | NM_002482.4 | TSL:1, GENCODE basic, MANE Select v0.92, |
| NASP-224 | ENST00000537798.5 | 3097 | 724aa | Protein coding | CCDS55597 | P49321-4 | - | TSL:2, GENCODE basic, |
| NASP-202 | ENST00000351223.7 | 1538 | 449aa | Protein coding | CCDS525 | P49321-2 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NASP-203 | ENST00000372052.8 | 1458 | 422aa | Protein coding | - | Q5T624 | - | TSL:5, GENCODE basic, |
| NASP-208 | ENST00000470768.5 | 1036 | 316aa | Protein coding | - | E9PPR5 | - | CDS 3' incomplete, TSL:3, |
| NASP-221 | ENST00000531612.5 | 938 | 288aa | Protein coding | - | H0YF33 | - | CDS 5' incomplete, TSL:5, |
| NASP-216 | ENST00000528238.5 | 894 | 268aa | Protein coding | - | E9PRH9 | - | CDS 3' incomplete, TSL:5, |
| NASP-205 | ENST00000437901.6 | 874 | 256aa | Protein coding | - | E9PI86 | - | CDS 3' incomplete, TSL:5, |
| NASP-223 | ENST00000534450.1 | 632 | 175aa | Protein coding | - | H0YDS9 | - | CDS 5' incomplete, TSL:2, |
| NASP-211 | ENST00000525515.5 | 565 | 154aa | Protein coding | - | E9PPQ8 | - | CDS 3' incomplete, TSL:5, |
| NASP-213 | ENST00000527470.5 | 532 | 143aa | Protein coding | - | E9PNB5 | - | CDS 3' incomplete, TSL:4, |
| NASP-225 | ENST00000629893.1 | 253 | 56aa | Protein coding | - | E9PKR5 | - | TSL:5, GENCODE basic, |
| NASP-206 | ENST00000453748.5 | 2080 | 38aa | Nonsense mediated decay | - | E9PAU3 | - | TSL:2, |
| NASP-204 | ENST00000437362.6 | 999 | 38aa | Nonsense mediated decay | - | E9PAU3 | - | TSL:2, |
| NASP-217 | ENST00000529333.5 | 778 | 38aa | Nonsense mediated decay | - | E9PAU3 | - | TSL:4, |
| NASP-212 | ENST00000527359.5 | 743 | 37aa | Nonsense mediated decay | - | E9PQG5 | - | TSL:3, |
| NASP-220 | ENST00000531532.5 | 554 | 56aa | Nonsense mediated decay | - | E9PKR5 | - | TSL:4, |
| NASP-215 | ENST00000528084.5 | 535 | 56aa | Nonsense mediated decay | - | E9PKR5 | - | TSL:4, |
| NASP-219 | ENST00000530840.5 | 531 | 55aa | Nonsense mediated decay | - | E9PJQ2 | - | TSL:5, |
| NASP-218 | ENST00000530073.5 | 2740 | No protein | Processed transcript | - | - | - | TSL:2, |
| NASP-222 | ENST00000534101.1 | 754 | No protein | Processed transcript | - | - | - | TSL:3, |
| NASP-210 | ENST00000481782.1 | 3659 | No protein | Retained intron | - | - | - | TSL:2, |
| NASP-214 | ENST00000527932.1 | 650 | No protein | Retained intron | - | - | - | TSL:3, |
| NASP-207 | ENST00000464190.6 | 604 | No protein | Retained intron | - | - | - | TSL:2, |
| NASP-209 | ENST00000472408.1 | 541 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NASP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NASP had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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