CRISPR-U™ Gene Knockout Cell Line Strategy

NASP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NASP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NASP
Gene id 4678
Organism Homo sapiens
Official full symbol nuclear autoantigenic sperm protein
Gene type protein-coding
Also known as FLB7527, HMDRA1, PRO1999
Summary This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
Genomic regions Chromosome 1
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NASP-201 ENST00000350030.8 3225 788aa Protein coding CCDS524 P49321-1 NM_002482.4 TSL:1, GENCODE basic, MANE Select v0.92,
NASP-224 ENST00000537798.5 3097 724aa Protein coding CCDS55597 P49321-4 - TSL:2, GENCODE basic,
NASP-202 ENST00000351223.7 1538 449aa Protein coding CCDS525 P49321-2 - TSL:1, GENCODE basic, APPRIS P1,
NASP-203 ENST00000372052.8 1458 422aa Protein coding - Q5T624 - TSL:5, GENCODE basic,
NASP-208 ENST00000470768.5 1036 316aa Protein coding - E9PPR5 - CDS 3' incomplete, TSL:3,
NASP-221 ENST00000531612.5 938 288aa Protein coding - H0YF33 - CDS 5' incomplete, TSL:5,
NASP-216 ENST00000528238.5 894 268aa Protein coding - E9PRH9 - CDS 3' incomplete, TSL:5,
NASP-205 ENST00000437901.6 874 256aa Protein coding - E9PI86 - CDS 3' incomplete, TSL:5,
NASP-223 ENST00000534450.1 632 175aa Protein coding - H0YDS9 - CDS 5' incomplete, TSL:2,
NASP-211 ENST00000525515.5 565 154aa Protein coding - E9PPQ8 - CDS 3' incomplete, TSL:5,
NASP-213 ENST00000527470.5 532 143aa Protein coding - E9PNB5 - CDS 3' incomplete, TSL:4,
NASP-225 ENST00000629893.1 253 56aa Protein coding - E9PKR5 - TSL:5, GENCODE basic,
NASP-206 ENST00000453748.5 2080 38aa Nonsense mediated decay - E9PAU3 - TSL:2,
NASP-204 ENST00000437362.6 999 38aa Nonsense mediated decay - E9PAU3 - TSL:2,
NASP-217 ENST00000529333.5 778 38aa Nonsense mediated decay - E9PAU3 - TSL:4,
NASP-212 ENST00000527359.5 743 37aa Nonsense mediated decay - E9PQG5 - TSL:3,
NASP-220 ENST00000531532.5 554 56aa Nonsense mediated decay - E9PKR5 - TSL:4,
NASP-215 ENST00000528084.5 535 56aa Nonsense mediated decay - E9PKR5 - TSL:4,
NASP-219 ENST00000530840.5 531 55aa Nonsense mediated decay - E9PJQ2 - TSL:5,
NASP-218 ENST00000530073.5 2740 No protein Processed transcript - - - TSL:2,
NASP-222 ENST00000534101.1 754 No protein Processed transcript - - - TSL:3,
NASP-210 ENST00000481782.1 3659 No protein Retained intron - - - TSL:2,
NASP-214 ENST00000527932.1 650 No protein Retained intron - - - TSL:3,
NASP-207 ENST00000464190.6 604 No protein Retained intron - - - TSL:2,
NASP-209 ENST00000472408.1 541 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NASP
This KO Strategy loading
Red Cotton™ Notes Gene NASP had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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