CRISPR-U™ Gene Knockout Cell Line Strategy

MYH2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MYH2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MYH2
Gene id 4620
Organism Homo sapiens
Official full symbol myosin heavy chain 2
Gene type protein-coding
Also known as IBM3, MYH2A, MYHSA2, MYHas8, MYPOP, MyHC-2A, MyHC-IIa
Summary Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Genomic regions Chromosome 17
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MYH2-202 ENST00000397183.6 6078 1941aa Protein coding CCDS11156 Q9UKX2-1 - TSL:5, GENCODE basic, APPRIS P1,
MYH2-201 ENST00000245503.10 6044 1941aa Protein coding CCDS11156 Q9UKX2-1 NM_017534.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MYH2-204 ENST00000532183.6 2528 708aa Protein coding - Q9UKX2-2 - TSL:1, GENCODE basic,
MYH2-206 ENST00000622564.4 2379 708aa Protein coding - Q9UKX2-2 - TSL:1, GENCODE basic,
MYH2-203 ENST00000420805.1 675 182aa Protein coding - E7EX84 - CDS 3' incomplete, TSL:5,
MYH2-205 ENST00000578017.5 572 155aa Protein coding - J3QLR0 - CDS 3' incomplete, TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MYH2
This KO Strategy loading
Red Cotton™ Notes Gene MYH2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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