CRISPR-U™ Gene Knockout Cell Line
Strategy
MYH2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MYH2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MYH2 |
| Gene id | 4620 |
| Organism | Homo sapiens |
| Official full symbol | myosin heavy chain 2 |
| Gene type | protein-coding |
| Also known as | IBM3, MYH2A, MYHSA2, MYHas8, MYPOP, MyHC-2A, MyHC-IIa |
| Summary | Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MYH2-202 | ENST00000397183.6 | 6078 | 1941aa | Protein coding | CCDS11156 | Q9UKX2-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| MYH2-201 | ENST00000245503.10 | 6044 | 1941aa | Protein coding | CCDS11156 | Q9UKX2-1 | NM_017534.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MYH2-204 | ENST00000532183.6 | 2528 | 708aa | Protein coding | - | Q9UKX2-2 | - | TSL:1, GENCODE basic, |
| MYH2-206 | ENST00000622564.4 | 2379 | 708aa | Protein coding | - | Q9UKX2-2 | - | TSL:1, GENCODE basic, |
| MYH2-203 | ENST00000420805.1 | 675 | 182aa | Protein coding | - | E7EX84 | - | CDS 3' incomplete, TSL:5, |
| MYH2-205 | ENST00000578017.5 | 572 | 155aa | Protein coding | - | J3QLR0 | - | CDS 3' incomplete, TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MYH2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MYH2 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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