CRISPR-U™ Gene Knockout Cell Line
Strategy
MYD88 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MYD88 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MYD88 |
| Gene id | 4615 |
| Organism | Homo sapiens |
| Official full symbol | MYD88 innate immune signal transduction adaptor |
| Gene type | protein-coding |
| Also known as | IMD68, MYD88D |
| Summary | This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MYD88-201 | ENST00000396334.8 | 2850 | 296aa | Protein coding | - | Q99836-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| MYD88-204 | ENST00000421516.3 | 2691 | 304aa | Protein coding | - | Q99836-6 | - | TSL:1, GENCODE basic, |
| MYD88-210 | ENST00000650905.2 | 2667 | 296aa | Protein coding | - | Q99836-1 | NM_002468.5 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MYD88-212 | ENST00000652213.1 | 2655 | 245aa | Protein coding | - | A0A494C0J8 | - | GENCODE basic, |
| MYD88-203 | ENST00000417037.8 | 2638 | 251aa | Protein coding | - | A0A0A0MSI9 | - | TSL:1, GENCODE basic, |
| MYD88-211 | ENST00000651800.2 | 2483 | 191aa | Protein coding | - | A0A3F2YP85 | - | GENCODE basic, |
| MYD88-209 | ENST00000650112.2 | 2352 | 146aa | Protein coding | - | A0A3B3ITY3 | - | GENCODE basic, |
| MYD88-207 | ENST00000481122.5 | 712 | No protein | Processed transcript | - | - | - | TSL:5, |
| MYD88-206 | ENST00000463956.1 | 583 | No protein | Processed transcript | - | - | - | TSL:4, |
| MYD88-208 | ENST00000484513.1 | 3314 | No protein | Retained intron | - | - | - | TSL:2, |
| MYD88-213 | ENST00000652590.1 | 2839 | No protein | Retained intron | - | - | - | - |
| MYD88-202 | ENST00000416282.3 | 2725 | No protein | Retained intron | - | - | - | TSL:1, |
| MYD88-205 | ENST00000460295.1 | 1088 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MYD88 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MYD88 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: