CRISPR-U™ Gene Knockout Cell Line Strategy

MYD88 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MYD88 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MYD88
Gene id 4615
Organism Homo sapiens
Official full symbol MYD88 innate immune signal transduction adaptor
Gene type protein-coding
Also known as IMD68, MYD88D
Summary This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 3
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MYD88-201 ENST00000396334.8 2850 296aa Protein coding - Q99836-1 - TSL:1, GENCODE basic, APPRIS P1,
MYD88-204 ENST00000421516.3 2691 304aa Protein coding - Q99836-6 - TSL:1, GENCODE basic,
MYD88-210 ENST00000650905.2 2667 296aa Protein coding - Q99836-1 NM_002468.5 GENCODE basic, APPRIS P1, MANE Select v0.92,
MYD88-212 ENST00000652213.1 2655 245aa Protein coding - A0A494C0J8 - GENCODE basic,
MYD88-203 ENST00000417037.8 2638 251aa Protein coding - A0A0A0MSI9 - TSL:1, GENCODE basic,
MYD88-211 ENST00000651800.2 2483 191aa Protein coding - A0A3F2YP85 - GENCODE basic,
MYD88-209 ENST00000650112.2 2352 146aa Protein coding - A0A3B3ITY3 - GENCODE basic,
MYD88-207 ENST00000481122.5 712 No protein Processed transcript - - - TSL:5,
MYD88-206 ENST00000463956.1 583 No protein Processed transcript - - - TSL:4,
MYD88-208 ENST00000484513.1 3314 No protein Retained intron - - - TSL:2,
MYD88-213 ENST00000652590.1 2839 No protein Retained intron - - - -
MYD88-202 ENST00000416282.3 2725 No protein Retained intron - - - TSL:1,
MYD88-205 ENST00000460295.1 1088 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MYD88
This KO Strategy loading
Red Cotton™ Notes Gene MYD88 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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