CRISPR-U™ Gene Knockout Cell Line Strategy

MX2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MX2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MX2
Gene id 4600
Organism Homo sapiens
Official full symbol MX dynamin like GTPase 2
Gene type protein-coding
Also known as MXB
Summary The protein encoded by this gene has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1.
Genomic regions Chromosome 21
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MX2-216 ENST00000680862.1 3509 715aa Protein coding CCDS13672 - - GENCODE basic, APPRIS P1,
MX2-205 ENST00000435611.6 3485 715aa Protein coding CCDS13672 C9JS04 - TSL:3, GENCODE basic, APPRIS P1,
MX2-201 ENST00000330714.8 3408 715aa Protein coding CCDS13672 P20592-1 NM_002463.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MX2-203 ENST00000416447.1 843 14aa Protein coding - C9J9T4 - CDS 3' incomplete, TSL:5,
MX2-206 ENST00000436410.5 582 129aa Protein coding - C9JZQ9 - CDS 3' incomplete, TSL:3,
MX2-204 ENST00000418103.2 1257 201aa Nonsense mediated decay - C9JEL4 - TSL:3,
MX2-208 ENST00000481838.5 562 96aa Nonsense mediated decay - - - CDS 5' incomplete, TSL:1,
MX2-218 ENST00000681460.1 1893 No protein Processed transcript - - - -
MX2-211 ENST00000494252.1 440 No protein Processed transcript - - - TSL:3,
MX2-212 ENST00000495892.1 4064 No protein Retained intron - - - TSL:2,
MX2-209 ENST00000482953.5 3595 No protein Retained intron - - - TSL:2,
MX2-215 ENST00000680539.1 3113 No protein Retained intron - - - -
MX2-207 ENST00000474368.1 2871 No protein Retained intron - - - TSL:2,
MX2-214 ENST00000680215.1 2366 No protein Retained intron - - - -
MX2-202 ENST00000398632.3 2211 No protein Retained intron - - - TSL:2,
MX2-217 ENST00000681171.1 2060 No protein Retained intron - - - -
MX2-210 ENST00000493753.2 1900 No protein Retained intron - - - TSL:3,
MX2-213 ENST00000496774.5 827 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MX2
This KO Strategy loading
Red Cotton™ Notes Gene MX2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: