CRISPR-U™ Gene Knockout Cell Line
Strategy
MX1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MX1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MX1 |
| Gene id | 4599 |
| Organism | Homo sapiens |
| Official full symbol | MX dynamin like GTPase 1 |
| Gene type | protein-coding |
| Also known as | IFI-78K, IFI78, MX, MxA, lncMX1-215 |
| Summary | This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 21 |
Strategy Summary
This gene has 36 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MX1-241 | ENST00000681415.1 | 3752 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-223 | ENST00000679626.1 | 3699 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-225 | ENST00000679911.1 | 3690 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-220 | ENST00000679464.1 | 3653 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-227 | ENST00000680182.1 | 3649 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-238 | ENST00000681191.1 | 3640 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-219 | ENST00000679445.1 | 3576 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-228 | ENST00000680347.1 | 3553 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-246 | ENST00000681867.1 | 3527 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-206 | ENST00000419044.6 | 3516 | 662aa | Protein coding | CCDS13673 | H9KVC3 | - | TSL:3, GENCODE basic, APPRIS P1, |
| MX1-245 | ENST00000681857.1 | 3488 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-203 | ENST00000398600.6 | 3470 | 662aa | Protein coding | CCDS13673 | P20591-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| MX1-237 | ENST00000681039.1 | 3428 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-234 | ENST00000680776.1 | 3325 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-229 | ENST00000680364.1 | 3310 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-222 | ENST00000679543.1 | 3309 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-226 | ENST00000680176.1 | 3302 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-243 | ENST00000681671.1 | 3298 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-242 | ENST00000681607.1 | 3289 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-239 | ENST00000681266.1 | 3288 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-224 | ENST00000679705.1 | 3256 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-244 | ENST00000681849.1 | 3251 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-230 | ENST00000680536.1 | 3205 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-233 | ENST00000680760.1 | 3203 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-231 | ENST00000680629.1 | 3144 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-247 | ENST00000681896.1 | 3125 | 662aa | Protein coding | CCDS13673 | - | - | GENCODE basic, APPRIS P1, |
| MX1-209 | ENST00000455164.6 | 2810 | 662aa | Protein coding | CCDS13673 | P20591-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| MX1-202 | ENST00000398598.8 | 2776 | 662aa | Protein coding | CCDS13673 | P20591-1 | NM_002462.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MX1-204 | ENST00000413778.6 | 2672 | 662aa | Protein coding | CCDS13673 | H9KVD0 | - | TSL:5, GENCODE basic, APPRIS P1, |
| MX1-205 | ENST00000417963.6 | 2303 | 662aa | Protein coding | CCDS13673 | H9KVC9 | - | TSL:5, GENCODE basic, APPRIS P1, |
| MX1-216 | ENST00000619682.1 | 1980 | 508aa | Protein coding | CCDS74796 | P20591-2 | - | TSL:5, GENCODE basic, |
| MX1-217 | ENST00000679386.1 | 3964 | 624aa | Protein coding | - | - | - | GENCODE basic, |
| MX1-201 | ENST00000288383.11 | 2777 | 654aa | Protein coding | - | F8W8T1 | - | TSL:2, GENCODE basic, |
| MX1-235 | ENST00000680942.1 | 2732 | 639aa | Protein coding | - | - | - | GENCODE basic, |
| MX1-207 | ENST00000424365.6 | 2701 | 609aa | Protein coding | - | H9KVC7 | - | TSL:5, GENCODE basic, |
| MX1-218 | ENST00000679408.1 | 2687 | 609aa | Protein coding | - | - | - | GENCODE basic, |
| MX1-248 | ENST00000681944.1 | 2724 | 480aa | Nonsense mediated decay | - | - | - | - |
| MX1-232 | ENST00000680637.1 | 5778 | No protein | Retained intron | - | - | - | - |
| MX1-240 | ENST00000681382.1 | 4675 | No protein | Retained intron | - | - | - | - |
| MX1-210 | ENST00000467510.2 | 4559 | No protein | Retained intron | - | - | - | TSL:3, |
| MX1-214 | ENST00000486275.2 | 3644 | No protein | Retained intron | - | - | - | TSL:5, |
| MX1-212 | ENST00000478268.1 | 3202 | No protein | Retained intron | - | - | - | TSL:2, |
| MX1-236 | ENST00000680980.1 | 1637 | No protein | Retained intron | - | - | - | - |
| MX1-221 | ENST00000679528.1 | 1402 | No protein | Retained intron | - | - | - | - |
| MX1-208 | ENST00000441677.6 | 1211 | No protein | Retained intron | - | - | - | TSL:4, |
| MX1-211 | ENST00000468506.5 | 752 | No protein | Retained intron | - | - | - | TSL:3, |
| MX1-215 | ENST00000491110.1 | 516 | No protein | Retained intron | - | - | - | TSL:1, |
| MX1-213 | ENST00000484465.1 | 388 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MX1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MX1 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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