CRISPR-U™ Gene Knockout Cell Line Strategy

MUC1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MUC1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MUC1
Gene id 4582
Organism Homo sapiens
Official full symbol mucin 1, cell surface associated
Gene type protein-coding
Also known as ADMCKD, ADMCKD1, CA 15-3, CD227, EMA, H23AG, KL-6, MAM6, MCD, MCKD, MCKD1, MUC-1, MUC-1/SEC, MUC-1/X, MUC1/ZD, PEM, PEMT, PUM
Summary This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 23 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MUC1-225 ENST00000612778.4 1836 484aa Protein coding CCDS72933 A0A087X2A4 - TSL:5, GENCODE basic, APPRIS ALT2,
MUC1-228 ENST00000620103.4 1811 475aa Protein coding CCDS55640 A0A0C4DGW3 - TSL:5, GENCODE basic, APPRIS ALT2,
MUC1-202 ENST00000338684.9 1107 241aa Protein coding CCDS72934 A0A0A0MRB3 - TSL:1, GENCODE basic,
MUC1-221 ENST00000610359.4 1101 239aa Protein coding CCDS72935 A0A087X0L2 - TSL:1, GENCODE basic,
MUC1-207 ENST00000368392.7 1032 264aa Protein coding CCDS30882 P15941-11 - TSL:1, GENCODE basic,
MUC1-227 ENST00000615517.4 951 189aa Protein coding CCDS72936 A6ZIE4 - TSL:1, GENCODE basic,
MUC1-208 ENST00000368393.7 937 198aa Protein coding CCDS55642 P15941-13 - TSL:1, GENCODE basic, APPRIS ALT2,
MUC1-201 ENST00000337604.6 859 273aa Protein coding CCDS1098 P15941-8 - TSL:1, GENCODE basic, APPRIS P4,
MUC1-206 ENST00000368390.7 804 255aa Protein coding CCDS30883 P15941-7 - TSL:1, GENCODE basic,
MUC1-210 ENST00000368398.7 729 230aa Protein coding CCDS55641 P15941-6 - TSL:1, GENCODE basic,
MUC1-204 ENST00000343256.9 648 203aa Protein coding CCDS41409 P15941-10 - TSL:1, GENCODE basic,
MUC1-209 ENST00000368396.8 516 159aa Protein coding CCDS41408 P15941-12 - TSL:1, GENCODE basic,
MUC1-223 ENST00000611571.4 4170 1262aa Protein coding - A0A087WWM1 - TSL:5, GENCODE basic, APPRIS ALT2,
MUC1-213 ENST00000462215.5 1189 135aa Protein coding - A0A087WWM2 - TSL:2, GENCODE basic,
MUC1-212 ENST00000457295.6 1161 268aa Protein coding - A0A0A0MSH4 - TSL:2, GENCODE basic, APPRIS ALT2,
MUC1-214 ENST00000462317.5 1112 228aa Protein coding - A0A087WVJ0 - CDS 5' incomplete, TSL:3,
MUC1-226 ENST00000614519.4 1093 123aa Protein coding - A0A087WZZ6 - TSL:2, GENCODE basic,
MUC1-224 ENST00000611577.4 1023 213aa Protein coding - A6ZIE3 - TSL:1, GENCODE basic,
MUC1-211 ENST00000438413.5 927 229aa Protein coding - B1AVQ7 - TSL:1, GENCODE basic,
MUC1-219 ENST00000485118.5 666 85aa Protein coding - A0A087WTR1 - CDS 5' incomplete, TSL:3,
MUC1-218 ENST00000471283.5 635 149aa Protein coding - Q7Z536 - TSL:1, GENCODE basic,
MUC1-205 ENST00000368389.6 543 168aa Protein coding - P15941-9 - TSL:1, GENCODE basic,
MUC1-203 ENST00000342482.8 537 166aa Protein coding - P15941-16 - TSL:1, GENCODE basic,
MUC1-222 ENST00000610468.4 1162 126aa Nonsense mediated decay - A0A087WZI8 - TSL:1,
MUC1-220 ENST00000498431.5 667 96aa Nonsense mediated decay - A0A087X061 - TSL:1,
MUC1-216 ENST00000467134.5 619 80aa Nonsense mediated decay - B6ECB5 - TSL:1,
MUC1-215 ENST00000466913.1 177 No protein Processed transcript - - - TSL:1,
MUC1-217 ENST00000468978.2 899 No protein Retained intron - - - TSL:2,
MUC1-229 ENST00000620770.1 572 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MUC1
This KO Strategy loading
Red Cotton™ Notes Gene MUC1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×