CRISPR-U™ Gene Knockout Cell Line Strategy

MT2A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MT2A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MT2A
Gene id 4502
Organism Homo sapiens
Official full symbol metallothionein 2A
Gene type protein-coding
Also known as MT-2, MT-II, MT2
Summary This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions, altering the intracellular concentration of heavy metals in the cell. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. The encoded protein interacts with the protein encoded by the homeobox containing 1 gene in some cell types, controlling intracellular zinc levels, affecting apoptotic and autophagy pathways. Some polymorphisms in this gene are associated with an increased risk of cancer.
Genomic regions Chromosome 16
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MT2A-201 ENST00000245185.6 401 61aa Protein coding CCDS10763 P02795 NM_005953.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MT2A-202 ENST00000561491.1 581 73aa Protein coding - H3BSP9 - TSL:2, GENCODE basic,
MT2A-204 ENST00000563985.1 688 No protein Processed transcript - - - TSL:2,
MT2A-205 ENST00000567300.1 416 No protein Processed transcript - - - TSL:2,
MT2A-203 ENST00000562017.1 903 No protein Retained intron - - - TSL:NA,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MT2A
This KO Strategy loading
Red Cotton™ Notes Gene MT2A had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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