CRISPR-U™ Gene Knockout Cell Line
Strategy
MT2A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MT2A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MT2A |
| Gene id | 4502 |
| Organism | Homo sapiens |
| Official full symbol | metallothionein 2A |
| Gene type | protein-coding |
| Also known as | MT-2, MT-II, MT2 |
| Summary | This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions, altering the intracellular concentration of heavy metals in the cell. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. The encoded protein interacts with the protein encoded by the homeobox containing 1 gene in some cell types, controlling intracellular zinc levels, affecting apoptotic and autophagy pathways. Some polymorphisms in this gene are associated with an increased risk of cancer. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MT2A-201 | ENST00000245185.6 | 401 | 61aa | Protein coding | CCDS10763 | P02795 | NM_005953.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MT2A-202 | ENST00000561491.1 | 581 | 73aa | Protein coding | - | H3BSP9 | - | TSL:2, GENCODE basic, |
| MT2A-204 | ENST00000563985.1 | 688 | No protein | Processed transcript | - | - | - | TSL:2, |
| MT2A-205 | ENST00000567300.1 | 416 | No protein | Processed transcript | - | - | - | TSL:2, |
| MT2A-203 | ENST00000562017.1 | 903 | No protein | Retained intron | - | - | - | TSL:NA, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MT2A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MT2A had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: