CRISPR-U™ Gene Knockout Cell Line Strategy

MPP2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MPP2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MPP2
Gene id 4355
Organism Homo sapiens
Official full symbol membrane palmitoylated protein 2
Gene type protein-coding
Also known as DLG2
Summary Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.
Genomic regions Chromosome 17
Strategy Summary
This gene has 20 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MPP2-219 ENST00000612133.4 4938 552aa Protein coding CCDS11471 D3DX48 - TSL:5, GENCODE basic, APPRIS P4,
MPP2-218 ENST00000536246.5 4351 541aa Protein coding CCDS62207 B4DZ84 - TSL:2, GENCODE basic,
MPP2-220 ENST00000622681.4 4242 541aa Protein coding CCDS62207 B4DZ84 - TSL:5, GENCODE basic,
MPP2-202 ENST00000377184.7 4206 569aa Protein coding CCDS62208 A0A0A0MRU0 - TSL:1, GENCODE basic, APPRIS ALT1,
MPP2-201 ENST00000269095.9 4202 552aa Protein coding CCDS11471 D3DX48 NM_005374.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
MPP2-203 ENST00000461854.5 3462 576aa Protein coding CCDS62209 D3DX49 - TSL:1, GENCODE basic,
MPP2-214 ENST00000523501.5 2121 541aa Protein coding CCDS62207 B4DZ84 - TSL:2, GENCODE basic,
MPP2-208 ENST00000520305.5 2111 413aa Protein coding CCDS62206 Q14168-5 - TSL:2, GENCODE basic,
MPP2-206 ENST00000518766.5 1896 597aa Protein coding CCDS62210 A0A0C4DH75 - TSL:2, GENCODE basic,
MPP2-221 ENST00000625880.2 4668 573aa Protein coding - A0A0D9SFF8 - TSL:5, GENCODE basic,
MPP2-213 ENST00000523220.5 857 189aa Protein coding - E5RI47 - CDS 3' incomplete, TSL:1,
MPP2-204 ENST00000473246.5 841 186aa Protein coding - A0A087WVY2 - CDS 3' incomplete, TSL:3,
MPP2-210 ENST00000520406.5 741 151aa Protein coding - E5RIU3 - CDS 3' incomplete, TSL:4,
MPP2-217 ENST00000524294.5 592 112aa Protein coding - A0A087X0C7 - CDS 3' incomplete, TSL:4,
MPP2-212 ENST00000522172.5 588 73aa Protein coding - E5RFN8 - CDS 3' incomplete, TSL:4,
MPP2-216 ENST00000523934.5 584 148aa Protein coding - E5RI32 - CDS 3' incomplete, TSL:4,
MPP2-215 ENST00000523762.5 564 140aa Protein coding - E5RJK0 - CDS 3' incomplete, TSL:5,
MPP2-211 ENST00000521178.5 545 126aa Protein coding - E5RK50 - CDS 3' incomplete, TSL:4,
MPP2-205 ENST00000518478.1 543 31aa Protein coding - E5RIM9 - CDS 3' incomplete, TSL:4,
MPP2-207 ENST00000520241.5 534 143aa Protein coding - E5RK44 - CDS 3' incomplete, TSL:4,
MPP2-209 ENST00000520319.1 802 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MPP2
This KO Strategy loading
Red Cotton™ Notes Gene MPP2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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