CRISPR-U™ Gene Knockout Cell Line
Strategy
MPP2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MPP2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MPP2 |
| Gene id | 4355 |
| Organism | Homo sapiens |
| Official full symbol | membrane palmitoylated protein 2 |
| Gene type | protein-coding |
| Also known as | DLG2 |
| Summary | Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 20 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MPP2-219 | ENST00000612133.4 | 4938 | 552aa | Protein coding | CCDS11471 | D3DX48 | - | TSL:5, GENCODE basic, APPRIS P4, |
| MPP2-218 | ENST00000536246.5 | 4351 | 541aa | Protein coding | CCDS62207 | B4DZ84 | - | TSL:2, GENCODE basic, |
| MPP2-220 | ENST00000622681.4 | 4242 | 541aa | Protein coding | CCDS62207 | B4DZ84 | - | TSL:5, GENCODE basic, |
| MPP2-202 | ENST00000377184.7 | 4206 | 569aa | Protein coding | CCDS62208 | A0A0A0MRU0 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| MPP2-201 | ENST00000269095.9 | 4202 | 552aa | Protein coding | CCDS11471 | D3DX48 | NM_005374.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| MPP2-203 | ENST00000461854.5 | 3462 | 576aa | Protein coding | CCDS62209 | D3DX49 | - | TSL:1, GENCODE basic, |
| MPP2-214 | ENST00000523501.5 | 2121 | 541aa | Protein coding | CCDS62207 | B4DZ84 | - | TSL:2, GENCODE basic, |
| MPP2-208 | ENST00000520305.5 | 2111 | 413aa | Protein coding | CCDS62206 | Q14168-5 | - | TSL:2, GENCODE basic, |
| MPP2-206 | ENST00000518766.5 | 1896 | 597aa | Protein coding | CCDS62210 | A0A0C4DH75 | - | TSL:2, GENCODE basic, |
| MPP2-221 | ENST00000625880.2 | 4668 | 573aa | Protein coding | - | A0A0D9SFF8 | - | TSL:5, GENCODE basic, |
| MPP2-213 | ENST00000523220.5 | 857 | 189aa | Protein coding | - | E5RI47 | - | CDS 3' incomplete, TSL:1, |
| MPP2-204 | ENST00000473246.5 | 841 | 186aa | Protein coding | - | A0A087WVY2 | - | CDS 3' incomplete, TSL:3, |
| MPP2-210 | ENST00000520406.5 | 741 | 151aa | Protein coding | - | E5RIU3 | - | CDS 3' incomplete, TSL:4, |
| MPP2-217 | ENST00000524294.5 | 592 | 112aa | Protein coding | - | A0A087X0C7 | - | CDS 3' incomplete, TSL:4, |
| MPP2-212 | ENST00000522172.5 | 588 | 73aa | Protein coding | - | E5RFN8 | - | CDS 3' incomplete, TSL:4, |
| MPP2-216 | ENST00000523934.5 | 584 | 148aa | Protein coding | - | E5RI32 | - | CDS 3' incomplete, TSL:4, |
| MPP2-215 | ENST00000523762.5 | 564 | 140aa | Protein coding | - | E5RJK0 | - | CDS 3' incomplete, TSL:5, |
| MPP2-211 | ENST00000521178.5 | 545 | 126aa | Protein coding | - | E5RK50 | - | CDS 3' incomplete, TSL:4, |
| MPP2-205 | ENST00000518478.1 | 543 | 31aa | Protein coding | - | E5RIM9 | - | CDS 3' incomplete, TSL:4, |
| MPP2-207 | ENST00000520241.5 | 534 | 143aa | Protein coding | - | E5RK44 | - | CDS 3' incomplete, TSL:4, |
| MPP2-209 | ENST00000520319.1 | 802 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MPP2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MPP2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: