CRISPR-U™ Gene Knockout Cell Line Strategy

NR3C2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NR3C2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NR3C2
Gene id 4306
Organism Homo sapiens
Official full symbol nuclear receptor subfamily 3 group C member 2
Gene type protein-coding
Also known as MCR, MLR, MR, NR3C2VIT
Summary This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 4
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NR3C2-203 ENST00000358102.8 5792 984aa Protein coding CCDS3772 P08235-1 NM_000901.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
NR3C2-202 ENST00000344721.8 5712 984aa Protein coding CCDS3772 P08235-1 - TSL:5, GENCODE basic, APPRIS P2,
NR3C2-208 ENST00000512865.5 3822 867aa Protein coding CCDS54811 P08235-4 - TSL:1, GENCODE basic,
NR3C2-209 ENST00000625323.2 5909 988aa Protein coding - P08235-3 - TSL:5, GENCODE basic, APPRIS ALT2,
NR3C2-207 ENST00000511528.1 2967 988aa Protein coding - P08235-3 - TSL:5, GENCODE basic, APPRIS ALT2,
NR3C2-201 ENST00000342437.8 3150 706aa Nonsense mediated decay - P08235-2 - TSL:2,
NR3C2-205 ENST00000503313.1 877 No protein Processed transcript - - - TSL:5,
NR3C2-206 ENST00000504753.1 438 No protein Processed transcript - - - TSL:5,
NR3C2-204 ENST00000503174.1 491 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NR3C2
This KO Strategy loading
Red Cotton™ Notes Gene NR3C2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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