CRISPR-U™ Gene Knockout Cell Line Strategy

MAP3K11 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAP3K11 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAP3K11
Gene id 4296
Organism Homo sapiens
Official full symbol mitogen-activated protein kinase kinase kinase 11
Gene type protein-coding
Also known as MEKK11, MLK-3, MLK3, PTK1, SPRK
Summary The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42.
Genomic regions Chromosome 11
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAP3K11-201 ENST00000309100.8 3543 847aa Protein coding CCDS8107 Q16584-1 NM_002419.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MAP3K11-208 ENST00000530153.5 2830 590aa Protein coding - Q16584-2 - TSL:2, GENCODE basic,
MAP3K11-210 ENST00000532507.5 1812 263aa Protein coding - E9PID4 - TSL:2, GENCODE basic,
MAP3K11-204 ENST00000526293.1 571 111aa Protein coding - E9PRQ2 - CDS 3' incomplete, TSL:4,
MAP3K11-207 ENST00000529839.1 561 96aa Protein coding - E9PLB1 - CDS 3' incomplete, TSL:4,
MAP3K11-202 ENST00000524848.5 2139 215aa Nonsense mediated decay - H0YCF5 - CDS 5' incomplete, TSL:2,
MAP3K11-213 ENST00000534432.1 664 No protein Processed transcript - - - TSL:3,
MAP3K11-206 ENST00000527304.1 5605 No protein Retained intron - - - TSL:NA,
MAP3K11-203 ENST00000524856.1 3787 No protein Retained intron - - - TSL:2,
MAP3K11-212 ENST00000534110.1 585 No protein Retained intron - - - TSL:4,
MAP3K11-205 ENST00000526647.1 582 No protein Retained intron - - - TSL:3,
MAP3K11-211 ENST00000533032.1 579 No protein Retained intron - - - TSL:3,
MAP3K11-209 ENST00000530949.1 546 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAP3K11
This KO Strategy loading
Red Cotton™ Notes Gene MAP3K11 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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