CRISPR-U™ Gene Knockout Cell Line
Strategy
MAP3K11 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAP3K11 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAP3K11 |
| Gene id | 4296 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase kinase kinase 11 |
| Gene type | protein-coding |
| Also known as | MEKK11, MLK-3, MLK3, PTK1, SPRK |
| Summary | The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAP3K11-201 | ENST00000309100.8 | 3543 | 847aa | Protein coding | CCDS8107 | Q16584-1 | NM_002419.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MAP3K11-208 | ENST00000530153.5 | 2830 | 590aa | Protein coding | - | Q16584-2 | - | TSL:2, GENCODE basic, |
| MAP3K11-210 | ENST00000532507.5 | 1812 | 263aa | Protein coding | - | E9PID4 | - | TSL:2, GENCODE basic, |
| MAP3K11-204 | ENST00000526293.1 | 571 | 111aa | Protein coding | - | E9PRQ2 | - | CDS 3' incomplete, TSL:4, |
| MAP3K11-207 | ENST00000529839.1 | 561 | 96aa | Protein coding | - | E9PLB1 | - | CDS 3' incomplete, TSL:4, |
| MAP3K11-202 | ENST00000524848.5 | 2139 | 215aa | Nonsense mediated decay | - | H0YCF5 | - | CDS 5' incomplete, TSL:2, |
| MAP3K11-213 | ENST00000534432.1 | 664 | No protein | Processed transcript | - | - | - | TSL:3, |
| MAP3K11-206 | ENST00000527304.1 | 5605 | No protein | Retained intron | - | - | - | TSL:NA, |
| MAP3K11-203 | ENST00000524856.1 | 3787 | No protein | Retained intron | - | - | - | TSL:2, |
| MAP3K11-212 | ENST00000534110.1 | 585 | No protein | Retained intron | - | - | - | TSL:4, |
| MAP3K11-205 | ENST00000526647.1 | 582 | No protein | Retained intron | - | - | - | TSL:3, |
| MAP3K11-211 | ENST00000533032.1 | 579 | No protein | Retained intron | - | - | - | TSL:3, |
| MAP3K11-209 | ENST00000530949.1 | 546 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAP3K11 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAP3K11 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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