CRISPR-U™ Gene Knockout Cell Line Strategy

MAP3K10 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAP3K10 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAP3K10
Gene id 4294
Organism Homo sapiens
Official full symbol mitogen-activated protein kinase kinase kinase 10
Gene type protein-coding
Also known as MEKK10, MLK2, MST
Summary The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.
Genomic regions Chromosome 19
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAP3K10-201 ENST00000253055.8 3754 954aa Protein coding CCDS12549 Q02779 NM_002446.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MAP3K10-207 ENST00000597986.5 2104 180aa Nonsense mediated decay - M0R1J8 - CDS 5' incomplete, TSL:5,
MAP3K10-202 ENST00000593502.5 739 122aa Nonsense mediated decay - M0R365 - CDS 5' incomplete, TSL:3,
MAP3K10-206 ENST00000594951.1 682 67aa Nonsense mediated decay - M0R0A7 - CDS 5' incomplete, TSL:2,
MAP3K10-203 ENST00000593906.1 555 No protein Processed transcript - - - TSL:5,
MAP3K10-205 ENST00000594791.1 899 No protein Retained intron - - - TSL:3,
MAP3K10-208 ENST00000601702.1 787 No protein Retained intron - - - TSL:3,
MAP3K10-204 ENST00000594569.1 562 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAP3K10
This KO Strategy loading
Red Cotton™ Notes Gene MAP3K10 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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