CRISPR-U™ Gene Knockout Cell Line
Strategy
MAP3K10 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAP3K10 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAP3K10 |
| Gene id | 4294 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase kinase kinase 10 |
| Gene type | protein-coding |
| Also known as | MEKK10, MLK2, MST |
| Summary | The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAP3K10-201 | ENST00000253055.8 | 3754 | 954aa | Protein coding | CCDS12549 | Q02779 | NM_002446.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MAP3K10-207 | ENST00000597986.5 | 2104 | 180aa | Nonsense mediated decay | - | M0R1J8 | - | CDS 5' incomplete, TSL:5, |
| MAP3K10-202 | ENST00000593502.5 | 739 | 122aa | Nonsense mediated decay | - | M0R365 | - | CDS 5' incomplete, TSL:3, |
| MAP3K10-206 | ENST00000594951.1 | 682 | 67aa | Nonsense mediated decay | - | M0R0A7 | - | CDS 5' incomplete, TSL:2, |
| MAP3K10-203 | ENST00000593906.1 | 555 | No protein | Processed transcript | - | - | - | TSL:5, |
| MAP3K10-205 | ENST00000594791.1 | 899 | No protein | Retained intron | - | - | - | TSL:3, |
| MAP3K10-208 | ENST00000601702.1 | 787 | No protein | Retained intron | - | - | - | TSL:3, |
| MAP3K10-204 | ENST00000594569.1 | 562 | No protein | Retained intron | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAP3K10 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAP3K10 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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