CRISPR-U™ Gene Knockout Cell Line
Strategy
MKI67 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MKI67 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MKI67 |
| Gene id | 4288 |
| Organism | Homo sapiens |
| Official full symbol | marker of proliferation Ki-67 |
| Gene type | protein-coding |
| Also known as | KIA, MIB-, MIB-1, PPP1R105 |
| Summary | This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MKI67-202 | ENST00000368654.8 | 12716 | 3256aa | Protein coding | CCDS7659 | P46013-1 | NM_002417.5 | TSL:2, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| MKI67-201 | ENST00000368653.7 | 11417 | 2896aa | Protein coding | CCDS53588 | P46013-2 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| MKI67-205 | ENST00000484853.1 | 733 | No protein | Processed transcript | - | - | - | TSL:2, |
| MKI67-203 | ENST00000464771.1 | 692 | No protein | Processed transcript | - | - | - | TSL:3, |
| MKI67-204 | ENST00000478293.1 | 654 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MKI67 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MKI67 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: