CRISPR-U™ Gene Knockout Cell Line Strategy

MKI67 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MKI67 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MKI67
Gene id 4288
Organism Homo sapiens
Official full symbol marker of proliferation Ki-67
Gene type protein-coding
Also known as KIA, MIB-, MIB-1, PPP1R105
Summary This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
Genomic regions Chromosome 10
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MKI67-202 ENST00000368654.8 12716 3256aa Protein coding CCDS7659 P46013-1 NM_002417.5 TSL:2, GENCODE basic, APPRIS P3, MANE Select v0.92,
MKI67-201 ENST00000368653.7 11417 2896aa Protein coding CCDS53588 P46013-2 - TSL:2, GENCODE basic, APPRIS ALT2,
MKI67-205 ENST00000484853.1 733 No protein Processed transcript - - - TSL:2,
MKI67-203 ENST00000464771.1 692 No protein Processed transcript - - - TSL:3,
MKI67-204 ENST00000478293.1 654 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MKI67
This KO Strategy loading
Red Cotton™ Notes Gene MKI67 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×