CRISPR-U™ Gene Knockout Cell Line Strategy

MEA1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MEA1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MEA1
Gene id 4201
Organism Homo sapiens
Official full symbol male-enhanced antigen 1
Gene type protein-coding
Also known as HYS, MEA
Genomic regions Chromosome 6
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MEA1-202 ENST00000642555.1 2152 172aa Protein coding CCDS87405 A0A2R8Y7W8 - GENCODE basic, APPRIS P1,
MEA1-201 ENST00000244711.4 2018 185aa Protein coding CCDS4879 Q16626 NM_014623.4 TSL:1, GENCODE basic, MANE Select v0.92,
MEA1-206 ENST00000645410.1 1355 172aa Protein coding CCDS87405 A0A2R8Y7W8 - GENCODE basic, APPRIS P1,
MEA1-203 ENST00000642748.1 1124 188aa Protein coding CCDS87406 A0A2R8Y6W5 - GENCODE basic,
MEA1-204 ENST00000643776.1 1004 172aa Protein coding CCDS87405 A0A2R8Y7W8 - GENCODE basic, APPRIS P1,
MEA1-205 ENST00000645375.1 978 172aa Protein coding CCDS87405 A0A2R8Y7W8 - GENCODE basic, APPRIS P1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MEA1
This KO Strategy loading
Red Cotton™ Notes Gene MEA1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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