CRISPR-U™ Gene Knockout Cell Line Strategy

MCL1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MCL1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MCL1
Gene id 4170
Organism Homo sapiens
Official full symbol MCL1 apoptosis regulator, BCL2 family member
Gene type protein-coding
Also known as BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT
Summary This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.
Genomic regions Chromosome 1
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MCL1-202 ENST00000369026.3 3950 350aa Protein coding CCDS957 Q07820-1 NM_021960.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
MCL1-205 ENST00000620947.4 3626 197aa Protein coding CCDS72909 A0A087WT64 - TSL:1, GENCODE basic, APPRIS ALT2,
MCL1-208 ENST00000678770.1 1256 350aa Protein coding CCDS957 - - GENCODE basic, APPRIS P2,
MCL1-201 ENST00000307940.3 1110 271aa Protein coding CCDS956 Q07820-2 - TSL:1, GENCODE basic,
MCL1-207 ENST00000678610.1 3801 152aa Protein coding - - - GENCODE basic,
MCL1-204 ENST00000617352.1 1036 No protein Processed transcript - - - TSL:5,
MCL1-203 ENST00000464132.2 4550 No protein Retained intron - - - TSL:2,
MCL1-206 ENST00000676522.1 4524 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MCL1
This KO Strategy loading
Red Cotton™ Notes Gene MCL1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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