CRISPR-U™ Gene Knockout Cell Line
Strategy
MCL1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MCL1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MCL1 |
| Gene id | 4170 |
| Organism | Homo sapiens |
| Official full symbol | MCL1 apoptosis regulator, BCL2 family member |
| Gene type | protein-coding |
| Also known as | BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT |
| Summary | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MCL1-202 | ENST00000369026.3 | 3950 | 350aa | Protein coding | CCDS957 | Q07820-1 | NM_021960.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| MCL1-205 | ENST00000620947.4 | 3626 | 197aa | Protein coding | CCDS72909 | A0A087WT64 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| MCL1-208 | ENST00000678770.1 | 1256 | 350aa | Protein coding | CCDS957 | - | - | GENCODE basic, APPRIS P2, |
| MCL1-201 | ENST00000307940.3 | 1110 | 271aa | Protein coding | CCDS956 | Q07820-2 | - | TSL:1, GENCODE basic, |
| MCL1-207 | ENST00000678610.1 | 3801 | 152aa | Protein coding | - | - | - | GENCODE basic, |
| MCL1-204 | ENST00000617352.1 | 1036 | No protein | Processed transcript | - | - | - | TSL:5, |
| MCL1-203 | ENST00000464132.2 | 4550 | No protein | Retained intron | - | - | - | TSL:2, |
| MCL1-206 | ENST00000676522.1 | 4524 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MCL1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MCL1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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