CRISPR-U™ Gene Knockout Cell Line
Strategy
MCAM Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MCAM Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MCAM |
| Gene id | 4162 |
| Organism | Homo sapiens |
| Official full symbol | melanoma cell adhesion molecule |
| Gene type | protein-coding |
| Also known as | CD146, HEMCAM, METCAM, MUC18, MelCAM |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MCAM-201 | ENST00000264036.6 | 3327 | 646aa | Protein coding | CCDS31690 | P43121-1 | NM_006500.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MCAM-214 | ENST00000530144.6 | 909 | No protein | Processed transcript | - | - | - | TSL:3, |
| MCAM-211 | ENST00000529295.5 | 757 | No protein | Processed transcript | - | - | - | TSL:4, |
| MCAM-209 | ENST00000528976.1 | 722 | No protein | Processed transcript | - | - | - | TSL:3, |
| MCAM-206 | ENST00000526992.1 | 574 | No protein | Processed transcript | - | - | - | TSL:4, |
| MCAM-217 | ENST00000534522.5 | 569 | No protein | Processed transcript | - | - | - | TSL:4, |
| MCAM-203 | ENST00000525555.5 | 567 | No protein | Processed transcript | - | - | - | TSL:4, |
| MCAM-210 | ENST00000529257.5 | 563 | No protein | Processed transcript | - | - | - | TSL:5, |
| MCAM-212 | ENST00000529686.5 | 563 | No protein | Processed transcript | - | - | - | TSL:4, |
| MCAM-215 | ENST00000530706.5 | 541 | No protein | Processed transcript | - | - | - | TSL:4, |
| MCAM-207 | ENST00000528502.5 | 478 | No protein | Processed transcript | - | - | - | TSL:4, |
| MCAM-202 | ENST00000524940.5 | 206 | No protein | Processed transcript | - | - | - | TSL:3, |
| MCAM-204 | ENST00000525586.5 | 4865 | No protein | Retained intron | - | - | - | TSL:5, |
| MCAM-208 | ENST00000528533.5 | 3164 | No protein | Retained intron | - | - | - | TSL:5, |
| MCAM-216 | ENST00000530937.5 | 588 | No protein | Retained intron | - | - | - | TSL:4, |
| MCAM-213 | ENST00000530006.5 | 562 | No protein | Retained intron | - | - | - | TSL:2, |
| MCAM-205 | ENST00000526190.5 | 555 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MCAM |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MCAM had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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