CRISPR-U™ Gene Knockout Cell Line
Strategy
MC2R Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MC2R Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MC2R |
| Gene id | 4158 |
| Organism | Homo sapiens |
| Official full symbol | melanocortin 2 receptor |
| Gene type | protein-coding |
| Also known as | ACTHR |
| Summary | MC2R encodes one member of the five-member G-protein associated melanocortin receptor family. Melanocortins (melanocyte-stimulating hormones and adrenocorticotropic hormone) are peptides derived from pro-opiomelanocortin (POMC). MC2R is selectively activated by adrenocorticotropic hormone, whereas the other four melanocortin receptors recognize a variety of melanocortin ligands. Mutations in MC2R can result in familial glucocorticoid deficiency. Alternate transcript variants have been found for this gene. |
| Genomic regions | Chromosome 18 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MC2R-201 | ENST00000327606.4 | 3652 | 297aa | Protein coding | CCDS11869 | Q01718 | NM_000529.2 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MC2R-202 | ENST00000399821.2 | 576 | 123aa | Protein coding | - | R4GMM0 | - | CDS 3' incomplete, TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MC2R |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MC2R had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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