CRISPR-U™ Gene Knockout Cell Line
Strategy
MATK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MATK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MATK |
| Gene id | 4145 |
| Organism | Homo sapiens |
| Official full symbol | megakaryocyte-associated tyrosine kinase |
| Gene type | protein-coding |
| Also known as | CHK, CTK, HHYLTK, HYL, HYLTK, Lsk |
| Summary | The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MATK-201 | ENST00000310132.11 | 2098 | 507aa | Protein coding | CCDS12114 | P42679-1 | NM_139355.3 | TSL:1, GENCODE basic, MANE Select v0.92, |
| MATK-203 | ENST00000395045.6 | 2073 | 508aa | Protein coding | CCDS12113 | P42679-2 | - | TSL:2, GENCODE basic, |
| MATK-202 | ENST00000395040.6 | 1907 | 466aa | Protein coding | CCDS42468 | P42679-3 | - | TSL:1, GENCODE basic, APPRIS P1, |
| MATK-215 | ENST00000619596.4 | 1740 | 508aa | Protein coding | CCDS12113 | P42679-2 | - | TSL:2, GENCODE basic, |
| MATK-204 | ENST00000585778.5 | 1994 | 506aa | Protein coding | - | K7EQY5 | - | TSL:1, GENCODE basic, |
| MATK-208 | ENST00000590493.5 | 801 | 267aa | Protein coding | - | K7ES68 | - | CDS 5' and 3' incomplete, TSL:3, |
| MATK-205 | ENST00000587180.1 | 741 | 131aa | Protein coding | - | K7ERY4 | - | CDS 3' incomplete, TSL:5, |
| MATK-207 | ENST00000590028.5 | 724 | 174aa | Protein coding | - | K7ENL8 | - | CDS 3' incomplete, TSL:4, |
| MATK-206 | ENST00000588983.5 | 711 | 237aa | Protein coding | - | K7EKS5 | - | CDS 5' and 3' incomplete, TSL:3, |
| MATK-212 | ENST00000591059.1 | 594 | 13aa | Protein coding | - | K7EKC4 | - | CDS 3' incomplete, TSL:4, |
| MATK-210 | ENST00000590849.1 | 571 | 84aa | Protein coding | - | K7EQV3 | - | CDS 3' incomplete, TSL:4, |
| MATK-211 | ENST00000590980.1 | 562 | 38aa | Protein coding | - | K7EPP2 | - | CDS 3' incomplete, TSL:4, |
| MATK-209 | ENST00000590821.1 | 659 | No protein | Processed transcript | - | - | - | TSL:3, |
| MATK-214 | ENST00000592612.1 | 575 | No protein | Processed transcript | - | - | - | TSL:4, |
| MATK-213 | ENST00000592300.1 | 544 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MATK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MATK had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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