CRISPR-U™ Gene Knockout Cell Line Strategy

MATK Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MATK Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MATK
Gene id 4145
Organism Homo sapiens
Official full symbol megakaryocyte-associated tyrosine kinase
Gene type protein-coding
Also known as CHK, CTK, HHYLTK, HYL, HYLTK, Lsk
Summary The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MATK-201 ENST00000310132.11 2098 507aa Protein coding CCDS12114 P42679-1 NM_139355.3 TSL:1, GENCODE basic, MANE Select v0.92,
MATK-203 ENST00000395045.6 2073 508aa Protein coding CCDS12113 P42679-2 - TSL:2, GENCODE basic,
MATK-202 ENST00000395040.6 1907 466aa Protein coding CCDS42468 P42679-3 - TSL:1, GENCODE basic, APPRIS P1,
MATK-215 ENST00000619596.4 1740 508aa Protein coding CCDS12113 P42679-2 - TSL:2, GENCODE basic,
MATK-204 ENST00000585778.5 1994 506aa Protein coding - K7EQY5 - TSL:1, GENCODE basic,
MATK-208 ENST00000590493.5 801 267aa Protein coding - K7ES68 - CDS 5' and 3' incomplete, TSL:3,
MATK-205 ENST00000587180.1 741 131aa Protein coding - K7ERY4 - CDS 3' incomplete, TSL:5,
MATK-207 ENST00000590028.5 724 174aa Protein coding - K7ENL8 - CDS 3' incomplete, TSL:4,
MATK-206 ENST00000588983.5 711 237aa Protein coding - K7EKS5 - CDS 5' and 3' incomplete, TSL:3,
MATK-212 ENST00000591059.1 594 13aa Protein coding - K7EKC4 - CDS 3' incomplete, TSL:4,
MATK-210 ENST00000590849.1 571 84aa Protein coding - K7EQV3 - CDS 3' incomplete, TSL:4,
MATK-211 ENST00000590980.1 562 38aa Protein coding - K7EPP2 - CDS 3' incomplete, TSL:4,
MATK-209 ENST00000590821.1 659 No protein Processed transcript - - - TSL:3,
MATK-214 ENST00000592612.1 575 No protein Processed transcript - - - TSL:4,
MATK-213 ENST00000592300.1 544 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MATK
This KO Strategy loading
Red Cotton™ Notes Gene MATK had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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