CRISPR-U™ Gene Knockout Cell Line Strategy

MAP4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAP4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAP4
Gene id 4134
Organism Homo sapiens
Official full symbol microtubule associated protein 4
Gene type protein-coding
Summary The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 3
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAP4-204 ENST00000395734.7 5590 1135aa Protein coding CCDS46818 P27816-6 - TSL:2, GENCODE basic, APPRIS ALT2,
MAP4-202 ENST00000360240.10 5142 1152aa Protein coding CCDS33750 P27816-1 - TSL:1, GENCODE basic, APPRIS ALT2,
MAP4-209 ENST00000434267.5 2772 99aa Protein coding CCDS46821 P27816-7 - TSL:2, GENCODE basic,
MAP4-210 ENST00000439356.2 2743 99aa Protein coding CCDS46821 P27816-7 - TSL:1, GENCODE basic,
MAP4-207 ENST00000426837.6 8920 2297aa Protein coding - E7EVA0 - TSL:5, GENCODE basic, APPRIS P3,
MAP4-208 ENST00000429422.5 3724 493aa Protein coding - H7C4C5 - CDS 5' incomplete, TSL:1,
MAP4-205 ENST00000420772.6 3478 828aa Protein coding - F8W9U4 - TSL:5, GENCODE basic, APPRIS ALT2,
MAP4-203 ENST00000383736.3 2282 760aa Protein coding - B5MEG9 - CDS 5' and 3' incomplete, TSL:5,
MAP4-201 ENST00000335271.9 1564 463aa Protein coding - H0Y2V1 - CDS 5' incomplete, TSL:2,
MAP4-206 ENST00000423088.5 899 283aa Protein coding - H7C456 - CDS 3' incomplete, TSL:3,
MAP4-216 ENST00000633710.1 570 140aa Protein coding - A0A0J9YW37 - CDS 3' incomplete, TSL:5,
MAP4-212 ENST00000468075.2 487 76aa Protein coding - A0A0J9YVV8 - CDS 3' incomplete, TSL:5,
MAP4-215 ENST00000497735.1 2156 No protein Retained intron - - - TSL:2,
MAP4-211 ENST00000462206.1 572 No protein Retained intron - - - TSL:4,
MAP4-214 ENST00000482752.1 386 No protein Retained intron - - - TSL:3,
MAP4-213 ENST00000477765.1 284 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAP4
This KO Strategy loading
Red Cotton™ Notes Gene MAP4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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