CRISPR-U™ Gene Knockout Cell Line
Strategy
MAGEA6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAGEA6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAGEA6 |
| Gene id | 4105 |
| Organism | Homo sapiens |
| Official full symbol | MAGE family member A6 |
| Gene type | protein-coding |
| Also known as | CT1.6, MAGE-3b, MAGE3B, MAGE6 |
| Summary | This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAGEA6-204 | ENST00000616035.4 | 1762 | 314aa | Protein coding | CCDS76050 | P43360 | - | TSL:5, GENCODE basic, APPRIS P1, |
| MAGEA6-201 | ENST00000329342.9 | 1740 | 314aa | Protein coding | CCDS76050 | P43360 | - | TSL:1, GENCODE basic, APPRIS P1, |
| MAGEA6-203 | ENST00000457643.1 | 921 | 235aa | Protein coding | - | E7ETG4 | - | CDS 3' incomplete, TSL:3, |
| MAGEA6-202 | ENST00000412733.1 | 862 | 201aa | Protein coding | - | E7EUF2 | - | CDS 3' incomplete, TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAGEA6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAGEA6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: